Lim Yong Taik, Lee Kun Yeong, Lee Kwangyeol, Chung Bong Hyun
Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Biochem Biophys Res Commun. 2006 Jun 9;344(3):926-30. doi: 10.1016/j.bbrc.2006.03.209. Epub 2006 Apr 19.
We described the development of functionalized magnetic nanoparticles (MNPs) with PEG-modification, a phospholipids micelle coating, and their use in manipulating histidine-tagged proteins. Highly monodisperse MNPs were synthesized in an organic solvent and could be phase-transferred into an aqueous solution by encapsulating the nanoparticles with a phospholipids micelle. The phospholipids micelle coating rendered the nanoparticles highly water-soluble, and the functional groups of the phospholipids coating allowed for the bioconjugation of various moieties, such as fluorescent molecules and engineered proteins. Functionalized phospholipids, such as nitrilotriacetic acid (NTA)-phospholipids, caused the MNPs to bind and allowed for manipulation of histidine-tagged proteins. Due to their high surface/volume ratio, the MNPs showed better performance (about 100 times higher) in immobilizing engineered proteins than conventional micrometer-sized beads. This demonstrates that MNPs coated with phospholipids micelle can be a versatile platform for the effective manipulation of various kinds of engineered proteins, which is very important in the field of proteomics. It is expected that a combination of MNPs with optical fluorescent molecules can find applications in bimodal (magnetic and optical) molecular imaging nanoprobes.
我们描述了具有聚乙二醇（PEG）修饰、磷脂胶束涂层的功能化磁性纳米颗粒（MNP）的开发及其在处理组氨酸标签蛋白中的应用。在有机溶剂中合成了高度单分散的MNP，通过用磷脂胶束包裹纳米颗粒可将其相转移到水溶液中。磷脂胶束涂层使纳米颗粒具有高度水溶性，并且磷脂涂层的官能团允许各种部分（如荧光分子和工程蛋白）进行生物共轭。功能化磷脂，如次氮基三乙酸（NTA）-磷脂，使MNP能够结合并处理组氨酸标签蛋白。由于其高表面/体积比，MNP在固定工程蛋白方面比传统的微米级珠子表现出更好的性能（约高100倍）。这表明涂有磷脂胶束的MNP可以成为有效处理各种工程蛋白的通用平台，这在蛋白质组学领域非常重要。预计MNP与光学荧光分子的组合可用于双模态（磁性和光学）分子成像纳米探针。
