基于菲咯啉的磁性纳米颗粒作为一种结合组氨酸标签蛋白的通用试剂。
Phenanthroline-based magnetic nanoparticles as a general agent to bind histidine-tagged proteins.
作者信息
Cho Eun Jin, Kim Hyun Joo, Song Younho, Choi In Seong, Bae Hyeun-Jong
机构信息
Department of Wood Science and Landscape Architecture, BK21 Program, Bio-energy Research Institute, Chonnam National University, Gwangju, 500-757, S. Korea.
出版信息
J Nanosci Nanotechnol. 2011 Aug;11(8):7104-7. doi: 10.1166/jnn.2011.4869.
We herein report the preparation of PSMN (phenanthroline-based silica coated magnetic nanoparticles), and their applications for protein purification as selective magnetic probes of histidine-tagged proteins in cell lysates. This simple system serves as a useful alternative to existing protocols for his-tagged protein separation and as a versatile agent for transporting and anchoring proteins.
我们在此报告了菲咯啉基二氧化硅包覆磁性纳米颗粒（PSMN）的制备及其作为细胞裂解物中组氨酸标签蛋白的选择性磁性探针用于蛋白质纯化的应用。这个简单的系统可作为现有组氨酸标签蛋白分离方案的有用替代方法，以及作为运输和锚定蛋白质的通用试剂。
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