鲑科各属间促性腺激素释放激素3（GNRH3）基因的比较。

Comparison of GNRH3 genes across salmonid genera.

作者信息

Leder E H, Danzmann R G, Ferguson M M

机构信息

Zoology Department, University of Guelph, Guelph, Ontario, Canada.

出版信息

Anim Genet. 2004 Apr;35(2):126-9. doi: 10.1111/j.1365-2052.2004.01104.x.

DOI:10.1111/j.1365-2052.2004.01104.x

PMID:15025573

Abstract

Genomic sequences of gonadotropin-releasing hormone genes were amplified and examined for sequence divergence among members of three different genera of the subfamily Salmoninae: rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar), and Arctic charr (Salvelinus alpinus). Sequences of GNRH3A and GNRH3B (formerly known as sGnRH1 and sGnRH2) were 97-99% similar in coding regions and 94-98% similar in non-coding regions among genera, but comparisons within species between GNRH3A and GNRH3B were only 90-92% similar in coding regions and 83-89% similar in non-coding regions. Polymorphisms in the parents of mapping families for each species allowed for linkage mapping of the GNRH3B gene in all three species and the GNRH3A gene in rainbow trout. GNRH3B maps to linkage group 6 in rainbow trout, linkage group 16 in Atlantic salmon and linkage group 25 in Arctic charr. GNRH3A mapped to linkage group 30 in rainbow trout.

摘要

对促性腺激素释放激素基因的基因组序列进行了扩增，并检测了鲑亚科三个不同属的成员之间的序列差异：虹鳟（Oncorhynchus mykiss）、大西洋鲑（Salmo salar）和北极红点鲑（Salvelinus alpinus）。促性腺激素释放激素3A（GNRH3A）和促性腺激素释放激素3B（GNRH3B，以前称为sGnRH1和sGnRH2）的序列在各属间的编码区相似度为97 - 99%，非编码区相似度为94 - 98%，但在种内GNRH3A和GNRH3B之间的编码区相似度仅为90 - 92%，非编码区相似度为83 - 89%。每个物种的作图家系亲本中的多态性使得在所有三个物种中对GNRH3B基因以及在虹鳟中对GNRH3A基因进行连锁作图成为可能。GNRH3B基因在虹鳟中定位于连锁群6，在大西洋鲑中定位于连锁群16，在北极红点鲑中定位于连锁群25。GNRH3A基因在虹鳟中定位于连锁群30。