Uhoda E, Goffin V, Pierard G E
Department of Dermatopathology, University Hospital of Liège, Liège, Belgium.
Contact Dermatitis. 2003 Dec;49(6):292-6. doi: 10.1111/j.0105-1873.2003.0269.x.
Skin is subjected to many environmental threats, some of which altering the structure and function of the stratum corneum. Among them, surfactants are recognized factors that may influence irritant contact dermatitis. The present study was conducted to compare the variations in skin capacitance and corneosurfametry (CSM) reactivity before and after skin exposure to repeated subclinical injuries by 2 hand dishwashing liquids. A forearm immersion test was performed on 30 healthy volunteers. 2 daily soak sessions were performed for 5 days. At inclusion and the day following the last soak session, skin capacitance was measured and cyanoacrylate skin-surface strippings were harvested. The latter specimens were used for the ex vivo microwave CSM. Both types of assessments clearly differentiated the 2 hand dishwashing liquids. The forearm immersion test allowed the discriminant sensitivity of CSM to increase. Intact skin capacitance did not predict CSM data. By contrast, a significant correlation was found between the post-test conductance and the corresponding CSM data. In conclusion, a forearm immersion test under realistic conditions can discriminate the irritation potential between surfactant-based products by measuring skin conductance and performing CSM. In vivo skin preconditioning by surfactants increases CSM sensitivity to the same surfactants.
皮肤面临多种环境威胁，其中一些会改变角质层的结构和功能。其中，表面活性剂是公认的可能影响刺激性接触性皮炎的因素。本研究旨在比较皮肤暴露于两种洗手液反复亚临床损伤前后皮肤电容和角质层表面活性测定（CSM）反应性的变化。对30名健康志愿者进行了前臂浸泡试验。每天浸泡2次，共进行5天。在纳入研究时以及最后一次浸泡后的当天，测量皮肤电容并采集氰基丙烯酸酯皮肤表面剥离物。后者的标本用于体外微波CSM。两种评估方法都清楚地区分了这两种洗手液。前臂浸泡试验使CSM的判别敏感性增加。完整皮肤电容无法预测CSM数据。相比之下，测试后的电导率与相应的CSM数据之间存在显著相关性。总之，在实际条件下进行的前臂浸泡试验可以通过测量皮肤电导率和进行CSM来区分基于表面活性剂的产品之间的刺激潜力。表面活性剂对皮肤进行的体内预处理会增加CSM对相同表面活性剂的敏感性。
