Yang Dong, Zhu You-Qing, Qi Jian
Department of Digestion, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, PR China.
Ai Zheng. 2004 Mar;23(3):306-9.
BACKGROUND & OBJECTIVE: The occurrence of colorectal adenocarcinoma is resulted from multiple factors and both survivin gene and PTEN protein take part in these courses. They all regulate cell cycle and apoptosis, but their biological effects are adverse. It is not completely elucidated about their function and relationship in colorectal adenocarcinoma. This study was designed to investigate the role of survivin gene and PTEN protein in the pathogenesis of colorectal adenocarcinoma and their correlation.
To determine the survivin mRNA in 42 colorectal adenocarcinoma and 20 adjacent normal colorectal tissue samples, we used reverse transcription polymerase chain reaction (RT-PCR) to determine the expression of survivin mRNA, and immunohistochemical evaluation was used to determine the expression of PTEN protein.
The positive expression rate of survivin was 54.8% in colorectal adenocarcinoma, while no expression was detected in adjacent normal tissue. The expression of survivin gene was significantly correlated with histological differentiation and Dukes stage, but not with gender or lymph node metastasis. The positive rate of PTEN protein in colorectal carcinoma was lower (47.6%) than that in adjacent normal tissue (90.0%). The difference between them was significant (P< 0.01). There was no relationship between PTEN protein and gender, while the expression of PTEN was positively correlated with histological differentiation and lymph node metastasis, and possibly correlated with Dukes stage. With the progress of tumor in malignancy, negative correlation was observed between survivin and PTEN expression in colorectal adenocarcinoma.
Survivin gene and PTEN protein are significantly correlated with the clinicopathological characteristics and biologic behaviors in colorectal adenocarcinoma. Detection of survivin together with PTEN is valuable for diagnosing colorectal adenocarcinoma, and evaluating malignancy extent and prognosis.
结直肠癌的发生是多种因素作用的结果，生存素基因和PTEN蛋白均参与其中。它们都对细胞周期和细胞凋亡起调控作用，但生物学效应相反。二者在结直肠癌中的作用及相互关系尚未完全阐明。本研究旨在探讨生存素基因和PTEN蛋白在结直肠癌发病机制中的作用及其相关性。
采用逆转录聚合酶链反应（RT-PCR）检测42例结直肠癌组织及20例癌旁正常结直肠组织中生存素mRNA的表达，免疫组织化学法检测PTEN蛋白的表达。
结直肠癌组织中生存素阳性表达率为54.8%，癌旁正常组织未检测到表达。生存素基因表达与组织学分级和Dukes分期显著相关，与性别及淋巴结转移无关。结直肠癌组织中PTEN蛋白阳性率（47.6%）低于癌旁正常组织（90.0%），差异有统计学意义（P<0.01）。PTEN蛋白表达与性别无关，与组织学分级和淋巴结转移呈正相关，与Dukes分期可能相关。随着肿瘤恶性程度增加，结直肠癌组织中生存素与PTEN表达呈负相关。
生存素基因和PTEN蛋白与结直肠癌的临床病理特征及生物学行为密切相关。联合检测生存素和PTEN对结直肠癌的诊断、评估恶性程度及预后具有重要价值。
