Mohammad Sameer, Taha Asia, Bamezai R N K, Basir Seemi Farhat, Baquer Najma Zaheer
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
Clin Chim Acta. 2004 Apr;342(1-2):105-14. doi: 10.1016/j.cccn.2003.12.005.
Vanadate treatment to diabetic rats has been reported to correct the altered carbohydrate metabolism and antioxidant status. However, vanadate exerts these effects at relatively high doses and several toxic effects are produced. We used low doses of vanadate in combination with Trigonella foenum graecum seed powder (TSP) and evaluated their effect on the enzyme changes in diabetic rats.
Alloxan-diabetic rats were treated separately with insulin, vanadate (0.6 mg/ml), TSP and a combined dose of Vanadate (0.2 mg/ml) and TSP for 21 days. At the end of the experimental period, blood glucose levels and activities of pyruvate kinase (PK), phosphoenolpyruvate carboxykinase (PEPCK), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) were measured in cytosolic fraction in the liver and kidney.
Blood glucose levels increased markedly in diabetic rats. Treatment with antidiabetic compounds resulted in the reduction of glucose levels. Rats treated with combined dose of vanadate and trigonella had glucose levels comparable to control ones. Similar results were obtained with the activities of PK, PEPCK, SOD, GPx, GR, and CAT in liver and kidney of diabetic rats. Combined dose of vanadate and Trigonella was found to be most effective in correcting these alterations.
Lower doses of vanadate could be used in combination with TSP to effectively counter diabetic alterations without any toxic side effects.
据报道，用钒酸盐治疗糖尿病大鼠可纠正其碳水化合物代谢和抗氧化状态的改变。然而，钒酸盐在相对高剂量时才发挥这些作用，并且会产生一些毒性作用。我们使用低剂量的钒酸盐与胡芦巴种子粉（TSP）联合使用，并评估它们对糖尿病大鼠酶变化的影响。
用胰岛素、钒酸盐（0.6毫克/毫升）、TSP以及钒酸盐（0.2毫克/毫升）与TSP的联合剂量分别治疗四氧嘧啶诱导的糖尿病大鼠21天。在实验期结束时，测量肝脏和肾脏胞质部分的血糖水平以及丙酮酸激酶（PK）、磷酸烯醇丙酮酸羧激酶（PEPCK）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。
糖尿病大鼠的血糖水平显著升高。用抗糖尿病化合物治疗可降低血糖水平。用钒酸盐和胡芦巴联合剂量治疗的大鼠血糖水平与对照组相当。在糖尿病大鼠肝脏和肾脏中PK、PEPCK、SOD、GPx、GR和CAT的活性也得到了类似结果。发现钒酸盐和胡芦巴的联合剂量在纠正这些改变方面最有效。
较低剂量的钒酸盐可与TSP联合使用，以有效对抗糖尿病改变，且无任何毒副作用。
