Salama A, Berghöfer H, Mueller-Eckhardt C
Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany.
Transfusion. 1992 Jul-Aug;32(6):554-6. doi: 10.1046/j.1537-2995.1992.32692367200.x.
Most drugs causing immunocytopenias do not bind firmly to the affected cells. Consequently, the drug-dependent antibodies in such cases are completely removed from their binding sites by conventional cell washing. It has recently been shown that such cell-drug-antibody complexes do survive the washing procedure, if the drug (metabolite) was included in the wash medium. The study reported here used the microtube gel test to reexamine the reactivity of different drug-dependent red cell antibodies: cefotaxime (n = 1), carbimazole (n = 1), cianidanol (n = 1), diclofenac (n = 3), penicillin (n = 3), and nomifensine (n = 10). Whether the drug tested binds (penicillin, cianidanol, carbimazole, and diclofenac) or does not bind (cefotaxime and nomifensine) firmly to the cells, the resultant cell-drug-antibody complex could be recognized on and/or in the gel after it was separated from the mixture containing the drug by means of centrifugation alone and without washing. It is concluded that the gel test might be of value not only for the detection of drug-dependent antibodies, but also for the analysis of subtle drug--cell interactions.
大多数引起免疫细胞减少的药物并不牢固地结合于受影响的细胞。因此，在这种情况下，药物依赖性抗体可通过常规细胞洗涤从其结合位点上完全去除。最近有研究表明，如果洗涤介质中含有药物（代谢物），这种细胞-药物-抗体复合物确实能在洗涤过程中存活下来。本研究采用微管凝胶试验重新检测了不同药物依赖性红细胞抗体的反应性：头孢噻肟（n = 1）、卡比马唑（n = 1）、西尼达醇（n = 1）、双氯芬酸（n = 3）、青霉素（n = 3）和诺米芬辛（n = 10）。无论所检测的药物（青霉素、西尼达醇、卡比马唑和双氯芬酸）是否牢固结合于细胞（头孢噻肟和诺米芬辛不结合），通过单独离心而不洗涤将细胞-药物-抗体复合物从含药物的混合物中分离后，均可在凝胶上和/或凝胶中识别出该复合物。得出的结论是，凝胶试验可能不仅对检测药物依赖性抗体有价值，而且对分析细微的药物-细胞相互作用也有价值。
