Li Shilin, Hu Liquan, Zhao Jiteng
Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China.
Zhonghua Nan Ke Xue. 2004 Feb;10(2):103-6.
To investigate the effects of growth hormone (GH) on the erectile function and the number of nNOS-containing nerve fibers in internal iliac arterial ligation rats.
Thirty-six mature male Wistar rats were randomized into 3 groups: GH intervention group, internal iliac arterial ligation group and sham operation group. After four weeks and eight weeks of treatment, one jalf of the rats of each group were selected respectively and tested for erectile function and then sacrificed for the detection of nNOS-containing nerve fibers by streptaridin-peroxidase immunohistochemistry techniques(SP method).
After four weeks, the erection frequency and the number of nNOS-containing never fibers in the sham operation group significantly increased compared with the other two groups, which showed no significant difference(P > 0.05). There was no significant difference in erection rate among the three groups. After eight weeks, the number of nNOS-containing never fibers in corpus carvosum in the internal iliac arterial ligation group decreased compared with the other two groups, between which there was no significant difference. And there was no significant difference in erection frequency and erection rate among the three groups.
Growth hormone can improve erectile function of internal iliac arterial ligation rats, which can be explained by the increase in the number of nNOS-containing never fibers in corpus carvosum of rats.
探讨生长激素（GH）对髂内动脉结扎大鼠勃起功能及含神经元型一氧化氮合酶（nNOS）神经纤维数量的影响。
将36只成年雄性Wistar大鼠随机分为3组：GH干预组、髂内动脉结扎组和假手术组。治疗4周和8周后，分别从每组中选取一半大鼠检测勃起功能，然后处死，采用链霉亲和素-过氧化物酶免疫组织化学技术（SP法）检测含nNOS神经纤维。
4周后，假手术组勃起频率及含nNOS神经纤维数量较其他两组显著增加，后两组间差异无统计学意义（P>0.05）。三组勃起率差异无统计学意义。8周后，髂内动脉结扎组阴茎海绵体含nNOS神经纤维数量较其他两组减少，后两组间差异无统计学意义。三组勃起频率和勃起率差异无统计学意义。
生长激素可改善髂内动脉结扎大鼠的勃起功能，其机制可能与大鼠阴茎海绵体含nNOS神经纤维数量增加有关。
