[利用分子标记鉴定和登记玉米基因型]

[Identification and registration of corn genotypes using molecular markers].

作者信息

Kozhukhova N E, Sivolap Iu M

机构信息

South Biotechnological Center of Plant Breeding, Ukrainian Academy of Agricultural Sciences, Odessa, 65036 Ukraine.

出版信息

Genetika. 2004 Jan;40(1):59-66.

PMID:15027201

Abstract

In plant genetics and breeding, second-generation molecular markers allow detailed characterization of plant genotypes. Unique genotypes at ten simple sequence repeat (SSR) loci were established for 40 maize accessions by means of PCR. For every locus, SSR analysis revealed heterozygotes among simple hybrids, which made it possible to identify the parental forms with a high probability of exclusion of nonparental forms. A system was proposed for registration of maize genotypes in the form of genetic formulas reflecting the allelic state of microsatellite loci, in order to catalog, preserve, and effectively employ the existing maize gene pool in breeding.

摘要

在植物遗传学和育种中，第二代分子标记可对植物基因型进行详细表征。通过聚合酶链式反应（PCR）为40份玉米种质建立了10个简单序列重复（SSR）位点的独特基因型。对于每个位点，SSR分析在简单杂种中揭示了杂合子，这使得识别亲本形式并以高概率排除非亲本形式成为可能。提出了一种以反映微卫星位点等位基因状态的遗传公式形式对玉米基因型进行登记的系统，以便在育种中编目、保存和有效利用现有的玉米基因库。