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细菌蛋白质转运：ATP和质子动力的动力学及热力学作用

Bacterial protein translocation: kinetic and thermodynamic role of ATP and the protonmotive force.

作者信息

Driessen A J

机构信息

Department of Microbiology, University of Groningen, Haren, The Netherlands.

出版信息

Trends Biochem Sci. 1992 Jun;17(6):219-23. doi: 10.1016/0968-0004(92)90381-i.

DOI:10.1016/0968-0004(92)90381-i
PMID:1502724
Abstract

The energetic mechanism of preprotein export in Escherichia coli has been a source of controversy for many years. In vitro studies of translocation reactions that use purified soluble and membrane components have not clarified the main features of this mechanism. Translocation occurs through consecutive steps which each have distinct energy requirements. Initiation of translocation requires ATP and the SecA protein. Most of the further steps can be driven by the protonmotive force (delta p).

摘要

多年来，大肠杆菌中前体蛋白输出的能量机制一直是争议的来源。使用纯化的可溶性和膜成分对转运反应进行的体外研究尚未阐明该机制的主要特征。转运通过连续步骤进行，每个步骤都有不同的能量需求。转运的起始需要ATP和SecA蛋白。大多数后续步骤可以由质子动力（Δp）驱动。

相似文献

1
Bacterial protein translocation: kinetic and thermodynamic role of ATP and the protonmotive force.细菌蛋白质转运：ATP和质子动力的动力学及热力学作用
Trends Biochem Sci. 1992 Jun;17(6):219-23. doi: 10.1016/0968-0004(92)90381-i.
2
The catalytic cycle of the escherichia coli SecA ATPase comprises two distinct preprotein translocation events.大肠杆菌SecA ATP酶的催化循环包括两个不同的前体蛋白转运事件。
EMBO J. 1997 Dec 15;16(24):7297-304. doi: 10.1093/emboj/16.24.7297.
3
Delta mu H+ and ATP function at different steps of the catalytic cycle of preprotein translocase.ΔμH⁺和ATP在前体蛋白转位酶催化循环的不同步骤中发挥作用。
Cell. 1991 Mar 8;64(5):927-39. doi: 10.1016/0092-8674(91)90317-r.
4
SecA protein: autoregulated ATPase catalysing preprotein insertion and translocation across the Escherichia coli inner membrane.SecA蛋白：一种自我调节的ATP酶，催化前体蛋白插入并转运穿过大肠杆菌内膜。
Mol Microbiol. 1993 Jan;7(2):159-65. doi: 10.1111/j.1365-2958.1993.tb01107.x.
5
The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation.纯化的大肠杆菌内膜蛋白SecY/E足以重建依赖SecA的前体蛋白易位。
Cell. 1990 Aug 24;62(4):649-57. doi: 10.1016/0092-8674(90)90111-q.
6
In vitro translocation of bacterial secretory proteins and energy requirements.
J Bioenerg Biomembr. 1990 Jun;22(3):389-99. doi: 10.1007/BF00763174.
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The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling.前体蛋白转运酶的SecDFyajC结构域通过调节SecA膜循环来控制前体蛋白的移动。
EMBO J. 1997 Aug 15;16(16):4871-9. doi: 10.1093/emboj/16.16.4871.
8
Proton transfer is rate-limiting for translocation of precursor proteins by the Escherichia coli translocase.质子转移是大肠杆菌转位酶转运前体蛋白的限速步骤。
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2471-5. doi: 10.1073/pnas.88.6.2471.
9
SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion.SecA通过经历由ATP驱动的膜插入和去插入循环来促进前体蛋白转运。
Cell. 1994 Sep 9;78(5):835-43. doi: 10.1016/s0092-8674(94)90582-7.
10
SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane.SecY和SecA相互作用，使SecA插入并使蛋白质穿过大肠杆菌质膜进行转运。
EMBO J. 1997 Nov 3;16(21):6384-93. doi: 10.1093/emboj/16.21.6384.

引用本文的文献

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J Am Chem Soc. 2013 Feb 27;135(8):2999-3010. doi: 10.1021/ja306361q. Epub 2013 Feb 14.
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The twin-arginine translocation (Tat) protein export pathway.双精氨酸转运（Tat）蛋白输出途径。
Nat Rev Microbiol. 2012 Jun 11;10(7):483-96. doi: 10.1038/nrmicro2814.
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Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion--tag.
旧蛋白的剖析揭示了新的应用：金黄色葡萄球菌蛋白 A（sSpAD）的结构域 D 作为一种分泌标签。
Microb Cell Fact. 2010 Nov 23;9:92. doi: 10.1186/1475-2859-9-92.
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The archaeal Sec-dependent protein translocation pathway.古菌依赖Sec的蛋白质转运途径。
Philos Trans R Soc Lond B Biol Sci. 2004 Jun 29;359(1446):919-27. doi: 10.1098/rstb.2003.1461.
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Thylakoid targeting of Tat passenger proteins shows no delta pH dependence in vivo.Tat 乘客蛋白的类囊体靶向在体内不显示 ΔpH 依赖性。
EMBO J. 2003 Feb 17;22(4):807-15. doi: 10.1093/emboj/cdg081.
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Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome.枯草芽孢杆菌中信号肽依赖性蛋白质转运：基于基因组的分泌蛋白组调查
Microbiol Mol Biol Rev. 2000 Sep;64(3):515-47. doi: 10.1128/MMBR.64.3.515-547.2000.
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Competition between Sec- and TAT-dependent protein translocation in Escherichia coli.大肠杆菌中依赖Sec和TAT的蛋白质转运之间的竞争
EMBO J. 1999 Jun 1;18(11):2982-90. doi: 10.1093/emboj/18.11.2982.
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The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation.在前体蛋白转运起始过程中，分子伴侣SecB从SecA的羧基末端释放出来。
EMBO J. 1997 Oct 15;16(20):6105-13. doi: 10.1093/emboj/16.20.6105.
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Secretion of nuclease across the outer membrane of Serratia marcescens and its energy requirements.粘质沙雷氏菌外膜核酸酶的分泌及其能量需求。
J Bacteriol. 1997 Feb;179(3):677-83. doi: 10.1128/jb.179.3.677-683.1997.
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Topology of the large envelope protein of duck hepatitis B virus suggests a mechanism for membrane translocation during particle morphogenesis.鸭乙型肝炎病毒大包膜蛋白的拓扑结构揭示了病毒颗粒形态发生过程中膜易位的机制。
J Virol. 1997 Feb;71(2):1107-14. doi: 10.1128/JVI.71.2.1107-1114.1997.