Choi Inchul, Oh Jungsu, Cho Byung-Nam, Ahnn Joohong, Jung Yong-Keun, Han Kim Do, Cho Chunghee
Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500-712, Republic of Korea.
Genomics. 2004 Apr;83(4):636-46. doi: 10.1016/j.ygeno.2003.10.001.
ADAM (a disintegrin and metalloprotease) family members with testis-specific or -predominant gene expression are divided phylogenically into two groups: ADAMs 2, 3, 5, 27, and 32 (the first group) and ADAMs 4, 6, 20, 21, 24, 25, 26, 29, 30, and 34 (the second group). We cloned and sequenced cDNAs for previously unidentified mouse Adams that belong to the second group. We found that all the Adam genes in the second phylogenic group are transcribed by both somatic and germ cells in mouse testis, representing a unique expression pattern different from that of the first-group Adams. Genomic analyses revealed that all the second-group Adam genes lack introns interrupting protein-coding sequences and many of them are present as multicopy genes, resulting in total of 14 functional mouse genes in this phylogenic group. Comparing the mouse and human ADAM genes, we found that a number of these mouse Adam genes do not have human orthologues and, even if they exist, some orthologues are pseudogenes in human. These results suggest the differential expansion of the second-group Adam genes in the mouse genome during evolution and a relationship between these Adams and male reproduction unique to mouse.
睾丸特异性或优势基因表达的ADAM（一种解整合素和金属蛋白酶）家族成员在系统发育上分为两组：ADAMs 2、3、5、27和32（第一组）以及ADAMs 4、6、20、21、24、25、26、29、30和34（第二组）。我们克隆并测序了属于第二组的此前未鉴定的小鼠Adam的cDNA。我们发现，系统发育第二组中的所有Adam基因在小鼠睾丸中均由体细胞和生殖细胞转录，这代表了一种与第一组Adam不同的独特表达模式。基因组分析显示，第二组所有Adam基因均缺乏中断蛋白质编码序列的内含子，其中许多以多拷贝基因形式存在，导致该系统发育组共有14个功能性小鼠基因。比较小鼠和人类的ADAM基因，我们发现许多这些小鼠Adam基因没有人类直系同源基因，即使存在，一些直系同源基因在人类中也是假基因。这些结果表明，在进化过程中第二组Adam基因在小鼠基因组中存在差异扩增，以及这些Adam与小鼠特有的雄性生殖之间的关系。
