Van Dael P, Shen L, Van Renterghem R, Deelstra H
Department of Pharmaceutical Science, University of Antwerp (UIA), Wilrijk, Belgium.
Z Lebensm Unters Forsch. 1992 Jul;195(1):3-7. doi: 10.1007/BF01197830.
This study reports on selenium distribution in goat milk. Skim milk was found to contain the major part (94%) of total milk selenium. The selenium distribution over casein and whey protein fractions depends on the separation method used, but irrespective of these methods, skim milk selenium is mainly associated with the casein fraction (greater than 69%). Approximately 9%, 7% and 24% of selenium is removed by dialysis (molecular cutoff 10-12 kDa) from skim milk, casein and whey respectively, indicating a major association of selenium with milk proteins. This observation is confirmed by selenium analysis of individual caseins and whey proteins isolated through ion-exchange chromatography and gel filtration. Selenium concentrations of the different isolated milk proteins show considerable variation (caseins: 294-550 ng Se/g; whey proteins: 217-457 ng Se/g).
本研究报告了山羊奶中硒的分布情况。发现脱脂奶中含有总奶硒的主要部分（94%）。酪蛋白和乳清蛋白组分中硒的分布取决于所采用的分离方法，但无论采用何种方法，脱脂奶中的硒主要与酪蛋白组分相关（大于69%）。通过透析（分子截留量10 - 12 kDa）分别从脱脂奶、酪蛋白和乳清中去除的硒约为9%、7%和24%，这表明硒主要与乳蛋白结合。通过离子交换色谱法和凝胶过滤法分离得到的单个酪蛋白和乳清蛋白的硒分析证实了这一观察结果。不同分离出的乳蛋白的硒浓度显示出相当大的差异（酪蛋白：294 - 550 ng硒/克；乳清蛋白：217 - 457 ng硒/克）。
