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羊奶中的硒含量及其在蛋白质组分中的分布。

Selenium content of sheep's milk and its distribution in protein fractions.

作者信息

Van Dael P, Shen L, Van Renterghem R, Deelstra H

机构信息

Department of Pharmaceutical Sciences, University of Antwerp (UIA), Wilrijk, Belgium.

出版信息

Z Lebensm Unters Forsch. 1993 Jun;196(6):536-9. doi: 10.1007/BF01201334.

DOI:10.1007/BF01201334
PMID:8328219
Abstract

The selenium (Se) distribution in sheep's milk is reported. Skim milk was found to contain the major part (98%) of total milk Se. The Se distribution over casein and whey protein fractions depends on the separation method used, but irrespective of these methods, skim milk Se is mainly associated with the casein fraction (> 68%). Approximately 11%, 4% and 17% Se is removed by dialysis (molecular mass cut-off 10-12 kDa) from skim milk, casein and whey respectively, indicating a major association of Se with milk proteins. This observation is confirmed through Se analysis of individual caseins and whey proteins isolated through ion-exchange chromatography and gel filtration. Se concentrations of the different isolated milk proteins show considerable variations (caseins, 304-750 ng Se/g; whey proteins, 241-806 ng Se/g).

摘要

报道了羊奶中硒(Se)的分布情况。发现脱脂奶中含有总奶硒的主要部分(98%)。酪蛋白和乳清蛋白组分中硒的分布取决于所采用的分离方法,但无论采用何种方法,脱脂奶中的硒主要与酪蛋白组分相关(>68%)。通过透析(截留分子量10 - 12 kDa)分别从脱脂奶、酪蛋白和乳清中去除的硒约为11%、4%和17%,这表明硒主要与乳蛋白结合。通过对通过离子交换色谱和凝胶过滤分离得到的单个酪蛋白和乳清蛋白进行硒分析,证实了这一观察结果。不同分离得到的乳蛋白的硒浓度显示出相当大的差异(酪蛋白,304 - 750 ng硒/克;乳清蛋白,241 - 806 ng硒/克)。

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