Ma John K, Zhu H E, Piletz John E
Department of Psychiatry, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA.
Ann N Y Acad Sci. 2003 Dec;1009:341-6. doi: 10.1196/annals.1304.043.
Immunoreactive proteins of 45-kD and 29/30-kD doublet bands are candidate imidazoline receptor binding proteins (IRBP) based on associations with I(1) or I(2) binding sites, respectively. It was reported that the density of cortical membrane 29/30-kD I(2) protein is diminished whereas a 45-kD I(1) protein is increased in depressed suicide victims versus controls. IRBP immunoreactive bands of similar size have been suggested to be breakdown products of the 170-kD protein known as IRAS (putative full-length I(1) receptor). This study compares nonpathologic human brains collected and frozen after postmortem delays of 13.4 hours +/- 1.7 (SEM) with brains of longer postmortem delays (26.1 hours +/- 1.2). The fresher human brains possessed more full-length IRAS (P = 0.05). In another study, the postmortem decay of IRBP bands in mouse brain was shown to be linear over time. The results are relevant to previous studies of IRBP bands in postmortem brains of depressed suicide victims.
基于分别与I(1)或I(2)结合位点的关联，45-kD和29/30-kD双峰带的免疫反应性蛋白是候选的咪唑啉受体结合蛋白(IRBP)。据报道，与对照组相比，抑郁自杀受害者的皮质膜29/30-kD I(2)蛋白密度降低，而45-kD I(1)蛋白增加。大小相似的IRBP免疫反应带被认为是170-kD蛋白IRAS(假定的全长I(1)受体)的分解产物。本研究将死后延迟13.4小时±1.7(SEM)后收集并冷冻的非病理性人脑与死后延迟较长(26.1小时±1.2)的人脑进行了比较。较新鲜的人脑拥有更多的全长IRAS(P = 0.05)。在另一项研究中，小鼠脑中IRBP带的死后衰变显示随时间呈线性。这些结果与之前关于抑郁自杀受害者死后大脑中IRBP带的研究相关。
