Piletz J E, Zhu H, Ordway G, Stockmeier C, Dilly G, Reis D, Halaris A
Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA.
Biol Psychiatry. 2000 Nov 1;48(9):910-9. doi: 10.1016/s0006-3223(00)00892-1.
A downregulation of I(2)-imidazoline binding sites has been reported in frontal cortices of depressed suicide victims, according to I(2)-radioligand binding and confirmed by Western blotting. We now report Western blots of imidazoline receptor proteins in hippocampi of subjects with and without depression at the time of death.
Postmortem diagnoses were obtained from 17 cases of Axis I major depressive disorder and 17 cases without Axis I psychopathology. No psychotropic compounds were found in body fluids. Hippocampi were removed, sectioned, and assessed histologically. Throughout the analysis, each major depressive disorder sample was paired with a sample from a psychiatrically healthy subject based on equivalent life spans and postmortem delays. The antiserum was identical to that used in previous studies that reported a downregulation of cortical 29/30-kd imidazoline receptor-binding proteins in depression.
A triad of imidazoline receptor-binding protein bands (40-50 kd) was detected in the human hippocampus. Subjects with major depressive disorder had significantly less intensity in each imidazoline receptor-binding proteins band compared with control subjects (p =. 01 for overall bands).
The present results can be aligned with previous reports of downregulation of I(2)-radioligand binding sites in both cortices and platelets of depressed patients.
根据I(2)-放射性配体结合实验报道，在抑郁自杀受害者的额叶皮质中，I(2)-咪唑啉结合位点下调，并通过蛋白质印迹法得到证实。我们现在报告死亡时患有和未患有抑郁症的受试者海马体中咪唑啉受体蛋白的蛋白质印迹结果。
从17例轴I型重度抑郁症患者和17例无轴I型精神病理学症状的患者中获取死后诊断结果。在体液中未发现精神活性化合物。取出海马体，切片并进行组织学评估。在整个分析过程中，根据相当的寿命和死后延迟时间，将每个重度抑郁症样本与来自精神健康受试者的样本配对。抗血清与先前研究中使用的相同，先前研究报道抑郁症患者皮质中29/30-kd咪唑啉受体结合蛋白下调。
在人类海马体中检测到一组三联咪唑啉受体结合蛋白条带（40-50 kd）。与对照组相比，重度抑郁症患者的每个咪唑啉受体结合蛋白条带的强度明显较低（所有条带的p =. 01）。
目前的结果与先前关于抑郁症患者皮质和血小板中I(2)-放射性配体结合位点下调的报道一致。
