[白蛋白与核糖核酸酶结合物的分离]

[Isolation of conjugates of albumin and ribonuclease].

作者信息

Zelepuga E A, Katushkina N V, Kolikov V M

机构信息

Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Vladivostok, 690022 Russia.

出版信息

Prikl Biokhim Mikrobiol. 2004 Jan-Feb;40(1):28-31.

PMID:15029693

Abstract

A method for separation of albumin-ribonuclease (RNase) conjugates has been proposed, based on the use of macroporous silicates. It was established that about 76% of ligand-free human serum albumin (LFHSA) formed complexes with enzymes. It was shown that most of the conjugates of albumin and pancreatic RNase contained up to 2 mol enzyme per 1 mol LFHSA. The conjugates of albumin and bacterial RNase, isolated from the cells of the strain Bacillus intermedius 7P, displayed higher specific activities, containing, on average, 2.3 mol RNase per 1 mol LFHSA (for the conjugates with molecular weights below 92 kDa) or 3.3 mol RNase per 1 mol protein carrier (for the conjugates with higher molecular weight).

摘要

已提出一种基于使用大孔硅酸盐分离白蛋白 - 核糖核酸酶（RNase）缀合物的方法。已确定约76%的无配体人血清白蛋白（LFHSA）与酶形成复合物。结果表明，白蛋白与胰腺RNase的大多数缀合物每1摩尔LFHSA含有多达2摩尔酶。从中间芽孢杆菌7P菌株细胞中分离得到的白蛋白与细菌RNase的缀合物表现出更高的比活性，平均而言，每1摩尔LFHSA含有2.3摩尔RNase（对于分子量低于92 kDa的缀合物）或每1摩尔蛋白质载体含有3.3摩尔RNase（对于分子量较高的缀合物）。