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[Isolation of intracellular inhibitors of bacterial RNAses on a column with immobilized Bacillus intermedius RNAse].

作者信息

Bannikova G E, Varlamov V P

出版信息

Prikl Biokhim Mikrobiol. 1994 Mar-Apr;30(3):379-83.

PMID:8047536
Abstract

Intracellular inhibitors of RNases from Bacillus amyloliquefaciens and Bac. intermedius were isolated using affinity chromatography on covalently immobilized RNase from Bac. intermedius. The inhibitor of RNase from Bac. amyloliquefaciens was isolated from cells of E. coli HB 101 and purified to homogeneity. Proteins with molecular weights of 70, 36 and 20 kD possessing an inhibitory activity were found in the extract obtained from frozen cells of Bac. intermedius.

摘要

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