Yakovlev Gennady I, Mitkevich Vladimir A, Struminskaya Nina K, Varlamov Valery P, Makarov Alexander A
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, Moscow 119991, Russia.
Biochem Biophys Res Commun. 2007 Jun 8;357(3):584-8. doi: 10.1016/j.bbrc.2007.03.033. Epub 2007 Mar 12.
RNase inhibitors are commonly used to block the RNase activity in manipulations with RNA-containing preparations. Recently RNase inhibitors, either synthetic or natural, have been intensively sought because they appeared to be promising for therapy of cancer and allergy. However, there is only a limited number of efficient RNase inhibitors. We have shown that a low molecular weight chitosan (M(r) approximately 6 kDa) inhibits activity of pancreatic RNase A and some bacterial RNases with inhibition constants in the range of 30-220 nM at pH 7.0 and ionic strength 0.14 M. The preferential contribution to the chitosan complex formation with RNases is due to establishment of 5-6 ion pairs. The results of this work show that polycations may efficiently inhibit ribonuclease activities.
核糖核酸酶抑制剂常用于在含RNA制剂的操作中阻断核糖核酸酶活性。近来，无论是合成的还是天然的核糖核酸酶抑制剂都受到了广泛关注，因为它们在癌症和过敏治疗方面似乎很有前景。然而，高效的核糖核酸酶抑制剂数量有限。我们已经表明，一种低分子量壳聚糖（相对分子质量约为6 kDa）在pH 7.0和离子强度0.14 M的条件下，能抑制胰腺核糖核酸酶A和一些细菌核糖核酸酶的活性，抑制常数在30 - 220 nM范围内。壳聚糖与核糖核酸酶形成复合物的优先贡献归因于5 - 6个离子对的形成。这项工作的结果表明，聚阳离子可以有效地抑制核糖核酸酶的活性。
