Shimazaki J, Laing R A, Tsubota K, Kenyon K R
Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Japan.
Nippon Ganka Gakkai Zasshi. 1992 Jul;96(7):828-33.
Redox states of the corneal endothelium in 42 recipient corneas obtained at the penetrating keratoplasty were measured non-invasively using ocular redox fluorometry. Autofluorescence from reduced pyridine nucleotides (PN) and oxidized flavoproteins (Fp) were measured, and the PN/Fp ratio was used as an indicator of the redox state. Endothelial damage was graded as normal, mildly damaged, moderately damaged, and severely damaged, based on the histopathological findings. Mildly damaged endothelium showed a significantly higher PN/Fp ratio than the that in normal endothelium whereas the ratio was significantly lower in the severely damaged endothelium. These changes in the redox state may represent compensation and decompensation processes of the endothelial metabolism. Ocular redox fluorometry was shown to be useful for the evaluation of the metabolic state in the human corneal endothelium.
利用眼氧化还原荧光测定法对42例穿透性角膜移植术中获取的受体角膜的角膜内皮氧化还原状态进行了无创测量。测量了还原型吡啶核苷酸（PN）和氧化型黄素蛋白（Fp）的自发荧光，并将PN/Fp比值用作氧化还原状态的指标。根据组织病理学结果，将内皮损伤分为正常、轻度损伤、中度损伤和重度损伤。轻度损伤的内皮PN/Fp比值显著高于正常内皮，而重度损伤的内皮该比值显著降低。氧化还原状态的这些变化可能代表内皮代谢的代偿和失代偿过程。结果表明，眼氧化还原荧光测定法可用于评估人角膜内皮的代谢状态。
