Shimazaki J, Laing R A, Tsubota K, Kenyon K R
Department of Ophthalmology, Boston University School of Medicine, USA.
Br J Ophthalmol. 1996 Jan;80(1):69-73. doi: 10.1136/bjo.80.1.69.
To investigate the usefulness of ocular redox fluorometry for evaluating donor corneal endothelial viability.
Corneas from 42 recipients of penetrating keratoplasty and four donor corneas were examined by ocular redox fluorometry. Autofluorescence from reduced pyridine nucleotides (PN) and oxidised flavoproteins (Fp) of the human corneal endothelium were measured non-invasively, and the PN/Fp ratio was used as a tissue metabolic indicator. Specular microscopy and electron microscopy were also performed.
Both the quality of specular microscopic image and the PN/Fp ratio were significantly correlated with the degree of corneal endothelial damage determined by histological examination. Corneas with poor specular microscopic image showed significantly decreased PN/Fp ratio compared with corneas with good or fair specular images (p = 0.041 and 0.027, respectively). The PN/Fp ratio increased in corneas with mildly damaged endothelium but decreased in corneas with severely damaged endothelium determined by histological examination. Evaluation of corneal endothelium by combination of specular microscopy and ocular redox fluorometry showed excellent association with that of histopathological examination (p < 0.0001).
Ocular redox fluorometry is useful for assessing donor corneal endothelial viability. Combination of ocular redox fluorometry and specular microscopy may increase the ability of donor cornea selection.
研究眼氧化还原荧光测定法在评估供体角膜内皮细胞活力方面的实用性。
对42例穿透性角膜移植受者的角膜以及4个供体角膜进行眼氧化还原荧光测定法检查。非侵入性地测量人角膜内皮细胞中还原型吡啶核苷酸（PN）和氧化型黄素蛋白（Fp）的自发荧光，并将PN/Fp比值用作组织代谢指标。同时进行角膜内皮显微镜检查和电子显微镜检查。
角膜内皮显微镜图像质量和PN/Fp比值均与组织学检查确定的角膜内皮损伤程度显著相关。与角膜内皮显微镜图像良好或中等的角膜相比，图像质量差的角膜PN/Fp比值显著降低（分别为p = 0.041和0.027）。根据组织学检查，内皮轻度损伤的角膜PN/Fp比值升高，而内皮严重损伤的角膜该比值降低。角膜内皮显微镜检查和眼氧化还原荧光测定法联合评估与组织病理学检查结果显示出极好的相关性（p < 0.0001）。
眼氧化还原荧光测定法有助于评估供体角膜内皮细胞活力。眼氧化还原荧光测定法与角膜内皮显微镜检查相结合可能会提高供体角膜选择的能力。
