Wang L, Chua H, Sin S N, Zhou Q, Ren D M, Li Z L
School of Life Sciences, Fudan University, Shanghai, PR China.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2004;39(1):223-35. doi: 10.1081/ese-120027380.
Heavy metal-containing wastewater is difficult to treat by most of the bioprocess due to its toxicity to microorganism in activated sludge. In this study, a combined bioprocess consisting of biosorption section that contained magnetite immobilized Pseudomonas putida 5-x cell as biosorbent followed by sequence batch reactor (SBR) was developed to treat Cu(2+)-containing municipal wastewater. The production techniques of Pseudomonas putida 5-x cell as biosorbent, such as optimal cell harvest period and cell pretreatment techniques were studied. Experimental results showed, considering both cell biomass and cell adsorption capacity to Cu(2+), 36 h is the optimal harvest period in the course of culturing Pseudomonas putida 5-x cells as biosorbent, and 0.1-0.3 mol L(-1) HCl is an optimal cell pretreating eluant to improve Cu(2+) adsorption capacity. The performances of the combined bioprocess for treating Cu(2+) containing wastewater were assessed. Experimental results showed that after treatment by biosorption, the Cu(2+) level in wastewater was reduced to level that did not inhibit COD removal efficiency of subsequent SBR activated sludge process, although it still affected the COD adsorption capacity of activated sludge. In terms of COD removal, the biosorption section was efficient for reducing Cu(2+) concentration to provide biodegradable wastewater for subsequent SBR activated sludge process.
