Hamuro J, Röllinghoff M, Wagner H
Cancer Res. 1978 Sep;38(9):3080-5.
Five different beta(1 leads to 3) glucans were tested for immune adjuvant activity on the in vivo induction of alloreactive murine cytotoxic T-lymphocytes (CTL). The beta(1 leads to 3) glucans, lentinan, pachyman, pachymaran, and two differently substitute hydroxyethylated pachymans strongly enhanced the in vivo generation of alloreactive CTL. The augmenting effect of i.p.-administered beta(1 leads to 3) glucans exhibited a clear dose-response relationship and was strictly dependent on the injection schedule used. Injection of high doses of beta(1 leads to 3) glucans as well as the injection during the late phase of the immune response markedly suppressed the magnitude of the lytic CTL activity induced. When the optimal conditions for enhanced CTL responses were chosen, the augmented CTL activity within spleen cells and mesenteric lymph node cells persisted for more than 25 days. Since beta(1 leads to 3) glucans are chemically defined substances without obvious toxic side effects, they may be of potential use to augment in vivo antigen-specific T-cell responses.
对五种不同的β（1→3）葡聚糖进行了测试，以研究其在体内诱导同种异体反应性小鼠细胞毒性T淋巴细胞（CTL）方面的免疫佐剂活性。β（1→3）葡聚糖、香菇多糖、茯苓聚糖、块菌多糖以及两种不同取代度的羟乙基化茯苓聚糖均能显著增强体内同种异体反应性CTL的生成。腹腔注射β（1→3）葡聚糖的增强作用呈现出明显的剂量反应关系，且严格依赖于所采用的注射方案。注射高剂量的β（1→3）葡聚糖以及在免疫反应后期进行注射，均显著抑制了所诱导的溶细胞性CTL活性的强度。当选择增强CTL反应的最佳条件时，脾细胞和肠系膜淋巴结细胞内增强的CTL活性可持续超过25天。由于β（1→3）葡聚糖是化学性质明确的物质，且无明显的毒副作用，它们可能具有增强体内抗原特异性T细胞反应的潜在用途。
