采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）结合薄层色谱（TLC）分析人和小型猪支气管肺泡灌洗（BAL）液的磷脂组成。

Analysis of the phospholipid composition of bronchoalveolar lavage (BAL) fluid from man and minipig by MALDI-TOF mass spectrometry in combination with TLC.

作者信息

Sommerer Daniela, Süss Rosmarie, Hammerschmidt Stefan, Wirtz Hubertus, Arnold Klaus, Schiller Jürgen

机构信息

Institute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Liebigstrasse 27, D-04103 Leipzig, Germany.

出版信息

J Pharm Biomed Anal. 2004 Apr 1;35(1):199-206. doi: 10.1016/j.jpba.2003.12.016.

DOI:10.1016/j.jpba.2003.12.016

PMID:15030895

Abstract

Surfaces of lungs are covered by the surfactant, an aqueous mixture of different phospholipids (PL) and proteins. Although the surfactant represents a relatively simple mixture of only a few PL (primarily phosphatidylcholine (PC) and phosphatidylglycerol (PG)), reliable methods of routine lipid analysis of the surfactant are still lacking. It will be shown that matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF MS) represents a suitable technique for the differentiation of the apolar components of the surfactant of different species. Samples of man and minipig are used in this study since both are known to vary in their PL composition. PL of surfactant were separated by thin-layer chromatography (TLC) and the obtained subfractions subjected to MALDI-TOF MS analysis in order to monitor the presence of even minor PL species. It will be shown that besides PG and PC, also phosphatidylethanolamine, -inositol and sphingomyelin can be detected in surfactant of man, whereas only sphingomyelin could be detected in the minipig sample.

摘要

肺表面覆盖有表面活性剂，它是不同磷脂（PL）和蛋白质的水性混合物。尽管表面活性剂仅由少数几种PL（主要是磷脂酰胆碱（PC）和磷脂酰甘油（PG））组成，是一种相对简单的混合物，但仍缺乏可靠的表面活性剂常规脂质分析方法。结果表明，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）是一种适用于区分不同物种表面活性剂中非极性成分的技术。本研究使用了人和小型猪的样本，因为已知两者的PL组成有所不同。表面活性剂的PL通过薄层色谱（TLC）分离，所得亚组分进行MALDI-TOF MS分析，以监测即使是微量PL种类的存在。结果表明，除了PG和PC外，在人表面活性剂中还可检测到磷脂酰乙醇胺、肌醇和鞘磷脂，而在小型猪样本中仅能检测到鞘磷脂。