Oktay Kutluk, Buyuk Erkan, Veeck Lucinda, Zaninovic Nikica, Xu Kangpu, Takeuchi Takumi, Opsahl Michael, Rosenwaks Zev
Center for Reproductive Medicine and Infertility, Joan and Sanford I Weill Medical College of Cornell University, 505 East 70th Street, HT-340, New York, NY 10021, USA.
Lancet. 2004 Mar 13;363(9412):837-40. doi: 10.1016/S0140-6736(04)15728-0.
Cancer treatments, including chemotherapy, radiotherapy, and radical surgery, can induce premature menopause and infertility in hundreds of thousands of women of reproductive age every year. One of the ways to possibly preserve fertility before these treatments is to cryopreserve ovarian tissue for later transplantation. We aimed to restore fertility by cryopreservation and transplantation of ovarian tissue.
Ovarian tissue was cryopreserved from a 30-year-old woman with breast cancer before chemotherapy-induced menopause, and this tissue was transplanted beneath the skin of her abdomen 6 years later.
Ovarian function returned in the patient 3 months after transplantation, as shown by follicle development and oestrogen production. The patient underwent eight oocyte retrievals percutaneously and 20 oocytes were retrieved. Of the eight oocytes suitable for in-vitro fertilisation, one fertilised normally and developed into a four-cell embryo.
Fertility and ovarian endocrine function can be preserved in women by long-term ovarian tissue banking.
癌症治疗，包括化疗、放疗和根治性手术，每年可导致数十万育龄妇女过早绝经和不孕。在这些治疗之前可能保留生育能力的方法之一是冷冻保存卵巢组织以供日后移植。我们旨在通过冷冻保存和移植卵巢组织来恢复生育能力。
在一名30岁乳腺癌女性化疗导致绝经前冷冻保存其卵巢组织，6年后将该组织移植到她腹部皮肤下。
移植3个月后患者卵巢功能恢复，表现为卵泡发育和雌激素分泌。患者经皮进行了8次卵母细胞采集，共采集到20个卵母细胞。在8个适合体外受精的卵母细胞中，1个正常受精并发育成4细胞胚胎。
通过长期的卵巢组织库保存，可保留女性的生育能力和卵巢内分泌功能。
