Jacobson S C, Guiochon G
Department of Chemistry, University of Tennessee, Knoxville 37996-1600.
Anal Chem. 1992 Jul 1;64(13):1496-8. doi: 10.1021/ac00037a032.
Bovine serum albumin (BSA) can be readily immobilized on ion-exchange stationary phases by frontal analysis of a proper solution. This provides a simple means of adjusting the amount of BSA contained in the column and of measuring it accurately. Although the immobilization is ionic and not covalent, the columns are stable for extensive periods of time. If needed, they can be easily regenerated by the same frontal analysis procedure.
通过对适当溶液进行前沿分析，牛血清白蛋白（BSA）可轻松固定在离子交换固定相上。这提供了一种调节柱中BSA含量并精确测量它的简单方法。尽管这种固定是离子性而非共价性的，但这些柱子在很长一段时间内都是稳定的。如有需要，可通过相同的前沿分析程序轻松使其再生。
