阿尔勒美利奴羊与布鲁拉美利奴羊杂交绵羊指纹的遗传分析

Genetic analysis of fingerprints in Mérinos d'Arles x Booroola Merino crossbred sheep.

作者信息

Lanneluc I, Hospital F, Chevalet C, Elsen J M, Gellin J

机构信息

INRA Centre de Recherches de Toulouse, Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France.

出版信息

Anim Genet. 1992;23(4):339-46. doi: 10.1111/j.1365-2052.1992.tb00156.x.

DOI:10.1111/j.1365-2052.1992.tb00156.x

PMID:1503273

Abstract

The M13.13 minisatellite probe, consisting of a polymer of the M13 VNTR consensus sequence, cross-hybridized to ovine DNA and allowed detection of several polymorphic loci. Individual specific patterns were obtained in sheep using this probe. Pedigree analysis showed that individuals were heterozygous for most of the DNA fragments detected (88%). By studying the segregation of male's variable DNA fragments, a minimum of 10 loci were defined. The ovine DNA 'fingerprint' obtained with M13.13 is polymorphic enough to be used efficiently in animal identification, paternity testing, and possibly as a source of genetic markers for linkage analysis.

摘要

M13.13小卫星探针由M13可变数目串联重复序列（VNTR）共有序列的聚合物组成，它与绵羊DNA发生交叉杂交，从而能够检测到多个多态性位点。使用该探针在绵羊中获得了个体特异性图谱。系谱分析表明，所检测到的大多数DNA片段（88%）在个体中是杂合的。通过研究雄性可变DNA片段的分离情况，确定了至少10个位点。用M13.13获得的绵羊DNA“指纹”具有足够的多态性，可有效地用于动物识别、亲子鉴定，并且可能作为连锁分析的遗传标记来源。