使用M13探针的绵羊DNA指纹图谱。

DNA fingerprints of sheep using an M13 probe.

作者信息

Gatei M H, Chen P M, Daniel R C, Lavin M F

机构信息

Department of Farm Animal Medicine and Production, University of Queensland, Brisbane, Australia.

出版信息

Anim Genet. 1991;22(3):285-9. doi: 10.1111/j.1365-2052.1991.tb00678.x.

DOI:10.1111/j.1365-2052.1991.tb00678.x

PMID:1928833

Abstract

The bacteriophage M13 DNA was used to detect hypervariable minisatellites in several families of Booroola sheep as well as Merino and Suffolk sheep. Digestion of sheep DNA gave rise to three to eight fragments with different restriction enzymes demonstrating considerable polymorphism between the different breeds. The length of informative DNA fragments varied in size from 6 to 20kb. The DNA fingerprints generated were individual specific and allowed for differentiation between closely related animals. The pattern obtained with sheep DNA was different from that observed with humans and other vertebrates in the proportion of high molecular weight DNA fragments present. Pedigree analysis of DNA patterns of dams and their offspring for several sets of twins and triplets showed a clear distinction between individuals and failed to reveal the presence of monozygosity.

摘要

噬菌体M13 DNA被用于检测布鲁拉羊以及美利奴羊和萨福克羊几个家族中的高变微卫星。用不同的限制性内切酶消化绵羊DNA会产生三到八个片段，这表明不同品种之间存在相当大的多态性。信息性DNA片段的长度在6到20kb之间变化。所产生的DNA指纹具有个体特异性，能够区分亲缘关系密切的动物。绵羊DNA产生的图谱与人类和其他脊椎动物的图谱不同，表现在高分子量DNA片段的比例上。对几组双胞胎和三胞胎的母羊及其后代的DNA图谱进行系谱分析，结果显示个体之间有明显区别，且未发现单合子的存在。