[结晶硫化镍诱导转化的人支气管上皮细胞基因组不稳定]
[Crystalline nickel sulfide-induced genomic instability in transformed human broncho-epithelial cells].
作者信息
Chen Chuan-de, Wu Zhong-liang, Chen Jia-kun, Ji Wei-dong
机构信息
Center for Disease Control and Prevention of Luohu District, Shenzhen 518020, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2004 Feb;22(1):57-9.
OBJECTIVE
To detect the genomic instability in the 16 human broncho-epithelial (16HBE) cells induced by crystalline nickel sulfide so as to provide the scientific basis for further study of nickel-induced cancer molecular mechanism.
METHODS
To analyse the genomic instability in transformed 16HBE cells induced by crystalline nickel sulfide by random amplified polymorphic DNA (RAPD).
RESULTS
All the 7 random primers selected could amplify 1 - 6 clear PCR bands. There were no significant differences between transformed 16HBE cells and negative control cells in the 4th, 5th, and 7th primers, but in the rest 4 primers there were significant differences, with special PCR bands for the same primer, indicating that genomic instability in transformed 16 HBE cells was induced by crystalline nickel sulfide.
CONCLUSION
Crystalline nickel sulfide could induce genomic instability in 16HBE cells.
目的
检测硫化镍晶体诱导人支气管上皮（16HBE）细胞的基因组不稳定性，为进一步研究镍致癌的分子机制提供科学依据。
方法
采用随机扩增多态性DNA（RAPD）技术分析硫化镍晶体诱导的转化16HBE细胞的基因组不稳定性。
结果
所选用的7条随机引物均能扩增出1～6条清晰的PCR条带。转化16HBE细胞与阴性对照细胞在第4、5、7条引物扩增结果上无明显差异，但在其余4条引物扩增结果上存在明显差异，同一引物出现特异PCR条带，提示硫化镍晶体可诱导转化16HBE细胞基因组不稳定性。
结论
硫化镍晶体可诱导16HBE细胞基因组不稳定性。
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