Manzanedo Carmen, Aguilar María A, Rodríguez-Arias Marta, Navarro Miguel, Miñarro José
Departamento de Psicobiología, Facultad de Psicología, Universidad Complutense de Madrid, 28223 Madrid, Spain.
Behav Brain Res. 2004 Apr 2;150(1-2):73-82. doi: 10.1016/S0166-4328(03)00225-0.
The effects of 7-nitroindazole (7-NI), a neural nitric oxide synthase (nNOS) inhibitor, on spontaneous locomotor activity, morphine-induced hyperactivity, acquisition of place conditioning and morphine-induced conditioned place preference (CPP) were evaluated in male mice. In experiment 1, animals treated with 7-NI (25, 50 and 100mg/kg), morphine (40 mg/kg) or morphine (40 mg/kg) plus 7-NI (25, 50 or 100mg/kg) were placed in an actimeter for 3h. In experiment 2, animals treated with the same drugs and doses were conditioned following an unbiased procedure. 7-NI did not affect the spontaneous locomotor activity or hyperactivity induced by morphine. However, the moderate and high doses of 7-NI produced conditioned place aversion (CPA) and the lowest dose blocked morphine-induced CPP. Our results suggest that nitric oxide is involved in the rewarding properties of morphine but not in its motor effects.
在雄性小鼠中评估了神经型一氧化氮合酶（nNOS）抑制剂7-硝基吲唑（7-NI）对自发运动活动、吗啡诱导的活动过度、位置条件反射的获得以及吗啡诱导的条件性位置偏爱（CPP）的影响。在实验1中，将接受7-NI（25、50和100毫克/千克）、吗啡（40毫克/千克）或吗啡（40毫克/千克）加7-NI（25、50或100毫克/千克）处理的动物放入活动计数器中3小时。在实验2中，对接受相同药物和剂量处理的动物按照无偏程序进行条件反射训练。7-NI不影响吗啡诱导的自发运动活动或活动过度。然而，中高剂量的7-NI产生了条件性位置厌恶（CPA），最低剂量则阻断了吗啡诱导的CPP。我们的结果表明，一氧化氮参与了吗啡的奖赏特性，但不参与其运动效应。
