骨髓基质细胞产生神经生长因子和胶质细胞系源性神经营养因子。
Bone marrow stromal cells produce nerve growth factor and glial cell line-derived neurotrophic factors.
作者信息
García Rocío, Aguiar Jorge, Alberti Esteban, de la Cuétara Karelys, Pavón Nancy
机构信息
International Center for Neurological Restoration, Havana City, Cuba.
出版信息
Biochem Biophys Res Commun. 2004 Apr 9;316(3):753-4. doi: 10.1016/j.bbrc.2004.02.111.
Bone marrow stromal cells (BMSC) have attracted interest through their possible use for cell therapy in neurological diseases. Recent reports demonstrated that these cells are able to migrate and have potential for neuronal differentiation after transplantation into brain parenchyma. The objective of this work was determine whether rat BMSC express NGF and GDNF, in order to study its potential application for treatment of neurodegenerative diseases. BMSC were harvested from male rats and cultured in DMEM supplemented with 20% fetal bovine serum. At passage 6 the total RNA was isolated using TriZol reactive. RT-PCRs to evaluate the expression of NGF and GDNF using specific primers were carried out. Our results indicate that rat BMSC have potential to produce NGF and GDNF. We have not found any report in favor of GDNF or NGF production from rat BMSC.
骨髓基质细胞（BMSC）因其在神经疾病细胞治疗中的潜在用途而备受关注。最近的报告表明，这些细胞能够迁移，并且在移植到脑实质后具有神经元分化的潜力。这项工作的目的是确定大鼠BMSC是否表达神经生长因子（NGF）和胶质细胞源性神经营养因子（GDNF），以便研究其在治疗神经退行性疾病中的潜在应用。从雄性大鼠中采集BMSC，并在补充有20%胎牛血清的DMEM中培养。在第6代时，使用TriZol试剂分离总RNA。使用特异性引物进行RT-PCR以评估NGF和GDNF的表达。我们的结果表明，大鼠BMSC具有产生NGF和GDNF的潜力。我们尚未发现任何支持大鼠BMSC产生GDNF或NGF的报告。
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