Kim Hark Kyun, Choi Il Ju, Kim Hee Sung, Kim Ju Han, Kim Eugene, Park In Sook, Chun Jong Ho, Kim In-Hoo, Kim Il-Jin, Kang Hio Chung, Park Jae-Hyun, Bae Jae-Moon, Lee Jin Soo, Park Jae-Gahb
Research Institute and Hospital, National Cancer Center, Goyang, Gyeonggi, Republic of Korea.
Biochem Biophys Res Commun. 2004 Apr 9;316(3):781-9. doi: 10.1016/j.bbrc.2004.02.109.
The mechanisms of intrinsic and/or acquired anti-cancer drug resistance have been described in in vitro resistance models, but the clinical relevance has remained undefined. We undertook a prospective study to identify correlations between gene expression and clinical resistance to 5-FU/cisplatin. We compared expression profiles from gastric cancer endoscopic biopsy specimens obtained at a chemosensitive state (partial remission after 5-FU/cisplatin) with those obtained at a refractory state (disease progression), using Affymetrix oligonucleotide microarray technology (U133A). Using 119 discriminating probes and a cross-validation approach, we were able to correctly identify the chemo-responsiveness of 7 pairs of training samples and 1 independent test pair. These exploratory data demonstrate that the gene expression profiles differ between chemosensitive and refractory state gastric cancer biopsy samples.
内在性和/或获得性抗癌药物耐药机制已在体外耐药模型中得到描述，但临床相关性仍不明确。我们进行了一项前瞻性研究，以确定基因表达与对5-氟尿嘧啶/顺铂临床耐药之间的相关性。我们使用Affymetrix寡核苷酸微阵列技术（U133A），比较了在化疗敏感状态（5-氟尿嘧啶/顺铂治疗后部分缓解）下获得的胃癌内镜活检标本与难治性状态（疾病进展）下获得的标本的表达谱。使用119个鉴别探针和交叉验证方法，我们能够正确识别7对训练样本和1对独立测试样本的化疗反应性。这些探索性数据表明，化疗敏感和难治性状态的胃癌活检样本之间的基因表达谱存在差异。
