通过DNA微阵列和Northern印迹分析对枯草芽孢杆菌中受SigD调控的基因进行系统分析。

Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.

作者信息

Serizawa Masakuni, Yamamoto Hiroki, Yamaguchi Hirotake, Fujita Yasutaro, Kobayashi Kazuo, Ogasawara Naotake, Sekiguchi Junichi

机构信息

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan.

出版信息

Gene. 2004 Mar 31;329:125-36. doi: 10.1016/j.gene.2003.12.024.

DOI:10.1016/j.gene.2003.12.024

PMID:15033535

Abstract

The SigD-regulated genes in Bacillus subtilis were systematically analyzed by comparing the pattern of transcripts derived from wild-type cells with those from sigD mutant cells using DNA microarray technology. One hundred and fifty-eight genes were found to be SigD-dependent candidates, 46 of which being known SigD-regulated genes. Northern blot analysis revealed that 18 of the remaining genes were SigD-dependent. The SigD consensus sequence was newly identified in the upstream regions of nine operons (11 genes): ybdO, yfmT-yfmS, hemAT, yjcP-yjcQ, yjfB, ylqB, yoaH, yscB and yxkC, and the other seven genes were assumed to be indirectly affected by a SigD mutation. Furthermore, yviE-yviF are likely to be SigD-dependent genes, because three independent sets of array data for yviE and yviF indicated they are SigD-dependent, and these genes are neighbors of flgL and hag transcribed by SigD RNA polymerase.

摘要

利用DNA微阵列技术，通过比较野生型细胞与sigD突变体细胞转录本的模式，对枯草芽孢杆菌中受SigD调控的基因进行了系统分析。发现有158个基因是SigD依赖性候选基因，其中46个是已知的受SigD调控的基因。Northern印迹分析表明，其余基因中有18个是SigD依赖性的。在9个操纵子（11个基因）的上游区域新鉴定出SigD共有序列：ybdO、yfmT-yfmS、hemAT、yjcP-yjcQ、yjfB、ylqB、yoaH、yscB和yxkC，另外7个基因被认为间接受到SigD突变的影响。此外，yviE-yviF可能是SigD依赖性基因，因为yviE和yviF的三组独立阵列数据表明它们是SigD依赖性的，并且这些基因是由SigD RNA聚合酶转录的flgL和hag的相邻基因。