Jervis Adrian J, Thackray Penny D, Houston Chris W, Horsburgh Malcolm J, Moir Anne
Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, England.
J Bacteriol. 2007 Jun;189(12):4534-8. doi: 10.1128/JB.00130-07. Epub 2007 Apr 13.
Promoters of nine Bacillus subtilis genes (bcrC, yacK, ydaH, yfnI, yjbD, ypbG, ypuA, yraA, and ysxA), all responsive to artificially induced increases in the stress-responsive extracytoplasmic function sigma factor, SigM, were mapped by rapid amplification of cDNA ends-PCR. The resulting promoter consensus suggests that overlapping control by SigX or SigW is common.
通过cDNA末端快速扩增PCR技术,对9个枯草芽孢杆菌基因(bcrC、yacK、ydaH、yfnI、yjbD、ypbG、ypuA、yraA和ysxA)的启动子进行了定位,这些基因均对胁迫应答胞质外功能σ因子SigM的人工诱导增加有反应。所得的启动子共有序列表明,SigX或SigW的重叠控制很常见。