Reszec Joanna, Sulkowska Mariola, Kanczuga-Koda Luiza, Janica Jerzy, Skawronska Malgorzata, Pepinski Witold, Sulkowski Stanislaw
Department of Clinical Pathology and Forensic Medicine, Medical Academy of Bialystok, Bialystok, Poland.
Ann N Y Acad Sci. 2003 Dec;1010:748-51. doi: 10.1196/annals.1299.135.
The balance between cell proliferation and programmed cell death plays a crucial role in malignant development. Bcl-2 family proteins, including proapoptosis protein Bak and antiapoptosis protein Bcl-2, regulate the apoptotic process. Mutation of the p53 gene, which results in P53 protein accumulation, was observed in many types of human cancer. The aim of our study was to evaluate immunohistochemical Bcl-2, Bak, and P53 protein expression and the relation between these proteins in conjunctival and eyelid benign and malignant tumors. We examined a series of 42 papillomas (CEP), 12 squamous cell cancers (SCC), and 19 cases of basal cell cancer (BCC). The age in the CEP group ranged from 18-94 years, and in the SCC and BCC groups from 42-87 years. Staining patterns were correlated with sex, age, and tumor localization. P53 protein-positive immunostaining was observed in 71% of cases, Bcl-2 in 83.9%, and Bak in 74.2 cases in the SCC and BCC groups. In the CEP group, P53 overexpression was observed in 90.5% of cases, Bcl-2 in 71.4%, and Bak in 76.2%. No statistically significant correlation was found between examined protein expression and sex, age, and tumor localization. An inverse correlation was observed between P53 and Bak protein expression in the CEP group. No statistically significance correlation was noted between Bcl-2 and P53 and Bcl-2 and Bak protein expression in both examined groups. The obtained data suggests that P53 and Bcl-2 protein expression coupled with decreasing Bak expression are associated with apoptosis and proliferation as well as malignant progression in conjunctival and eyelid tumors.
细胞增殖与程序性细胞死亡之间的平衡在恶性肿瘤发展过程中起着至关重要的作用。Bcl-2家族蛋白，包括促凋亡蛋白Bak和抗凋亡蛋白Bcl-2，调节细胞凋亡过程。在多种人类癌症中都观察到了p53基因的突变，这会导致P53蛋白积累。我们研究的目的是评估免疫组织化学检测结膜和眼睑良恶性肿瘤中Bcl-2、Bak和P53蛋白的表达情况以及这些蛋白之间的关系。我们检查了一系列42例结膜乳头状瘤（CEP）、12例鳞状细胞癌（SCC）和19例基底细胞癌（BCC）。CEP组患者年龄在18至94岁之间，SCC组和BCC组患者年龄在42至87岁之间。染色模式与性别、年龄和肿瘤定位相关。在SCC组和BCC组中，71%的病例观察到P53蛋白阳性免疫染色，83.9%的病例观察到Bcl-2阳性，74.2%的病例观察到Bak阳性。在CEP组中，90.5%的病例观察到P53过表达，71.4%的病例观察到Bcl-2过表达，76.2%的病例观察到Bak过表达。在所检测的蛋白表达与性别、年龄和肿瘤定位之间未发现统计学显著相关性。在CEP组中观察到P53与Bak蛋白表达呈负相关。在两个检测组中，未观察到Bcl-2与P53以及Bcl-2与Bak蛋白表达之间存在统计学显著相关性。所获得的数据表明，P53和Bcl-2蛋白表达以及Bak表达的降低与结膜和眼睑肿瘤的细胞凋亡、增殖以及恶性进展相关。
