Vojta Aleksandar, Alavi Marcel, Becker Thomas, Hörmann Friederike, Küchler Michael, Soll Jürgen, Thomson Rowena, Schleiff Enrico
Botanik, LMU München, Menzinger Strasse 67, 80638 Münich, Germany.
J Biol Chem. 2004 May 14;279(20):21401-5. doi: 10.1074/jbc.M401968200. Epub 2004 Mar 21.
The Toc and Tic translocon facilitate import of preproteins into chloroplasts. In the past, it was speculated that several translocon subunits act specifically for different types of precursor proteins or in different tissues. To generate a comprehensive picture of the expression and tissue-specific localization of the translocon subunits, their transcript levels were analyzed in roots and leaves. Certain Tocs and Tics were found to be tissue-specific. The protein composition of the transloci in the envelope membranes of chloroplasts was analyzed to describe the function and possible stoichiometry. In contrast to Tic subunits, several Toc subunits seem to have a high turnover.
Toc和Tic转位子有助于前体蛋白导入叶绿体。过去曾推测，几个转位子亚基专门作用于不同类型的前体蛋白或在不同组织中发挥作用。为了全面了解转位子亚基的表达和组织特异性定位，分析了它们在根和叶中的转录水平。发现某些Toc和Tic具有组织特异性。分析了叶绿体包膜膜中转位子的蛋白质组成，以描述其功能和可能的化学计量。与Tic亚基不同，几个Toc亚基似乎具有较高的周转率。
