Teel R W
Department of Physiology and Pharmacology, School of Medicine, Loma Linda Health Sciences University, CA 92350.
Anticancer Res. 1992 Jul-Aug;12(4):1287-90.
The effects of six inducers of isoenzymes of cytochrome P450 on the mutagenicity of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in Salmonella typhimurium strain TA1535 by hamster liver S9 and microsomes were investigated. Comparisons of the effects of dimethylsulfoxide (DMSO) as solvent for NNK were also made. The inducing agents were Aroclor 1254 (AROC), 3-methylcholanthrene (MC), phenobarbital (PB), dexamethasone (DXM), ethanol (ETOH) and isosafrol (ISF). The number of histidine-independent colonies induced by NNK in saline mediated by S9 from the inducing agents was as follows: ISF = AROC greater than PB greater than MC greater than DXM greater than ETOH. AROC-induced microsomes produced the most revertants by NNK (saline) greater than MC greater than PB = DXM and ISF greater than ETOH. The number of revertant colonies induced by NNK in DMSO was significantly less than that by NNK in saline for both hamster liver S9 and microsomes irrespective of the inducing agent. The greatest inhibitory effect of DMSO was observed with ISF-induced S9.
研究了细胞色素P450同工酶的六种诱导剂对仓鼠肝脏S9和微粒体在鼠伤寒沙门氏菌TA1535菌株中对烟草特异性亚硝胺4-(甲基亚硝氨基)-1-(3-吡啶基)-1-丁酮(NNK)诱变性的影响。还比较了二甲基亚砜(DMSO)作为NNK溶剂的影响。诱导剂为多氯联苯混合物1254(AROC)、3-甲基胆蒽(MC)、苯巴比妥(PB)、地塞米松(DXM)、乙醇(ETOH)和异黄樟素(ISF)。由诱导剂诱导产生的S9在盐溶液中由NNK诱导的组氨酸非依赖菌落数如下：ISF = AROC大于PB大于MC大于DXM大于ETOH。AROC诱导的微粒体由NNK产生的回复突变体最多(盐溶液)大于MC大于PB = DXM和ISF大于ETOH。对于仓鼠肝脏S9和微粒体，无论诱导剂如何，NNK在DMSO中诱导的回复菌落数均显著少于NNK在盐溶液中诱导的回复菌落数。在ISF诱导的S9中观察到DMSO的最大抑制作用。
