Kolde G, Schulze-Osthoff K, Meyer H, Knop J
Department of Dermatology, University of Münster, Federal Republic of Germany.
Arch Dermatol Res. 1992;284(3):154-8. doi: 10.1007/BF00372709.
The presence, distribution and cellular localization of tumour necrosis factor-alpha (TNF alpha) were investigated in normal human and murine epidermis using immunohistological and immunoelectron microscopic methods with monoclonal and polyclonal antibodies. The immunostaining revealed an intercellular plasma membrane and cytoplasmic labelling of the epidermal keratinocytes, but no labelling of Langerhans cells, melanocytes and Merkel cells. Large amounts of TNF alpha were regularly found in the sebaceous glands. These findings demonstrate that epidermal keratinocytes and especially sebocytes produce and release TNF alpha and that this keratinocyte-derived cytokine may be important for the structural and functional homeostasis of normal epidermis.
利用单克隆抗体和多克隆抗体，通过免疫组织学和免疫电子显微镜方法，研究了肿瘤坏死因子-α（TNFα）在正常人和小鼠表皮中的存在、分布及细胞定位。免疫染色显示，表皮角质形成细胞的细胞间质膜和细胞质有标记，但朗格汉斯细胞、黑素细胞和默克尔细胞无标记。皮脂腺中经常发现大量TNFα。这些发现表明，表皮角质形成细胞尤其是皮脂腺细胞产生并释放TNFα，这种角质形成细胞衍生的细胞因子可能对正常表皮的结构和功能稳态很重要。
