Suhling Klaus, Siegel Jan, Lanigan Peter M P, Lévêque-Fort Sandrine, Webb Stephen E D, Phillips David, Davis Daniel M, French Paul M W
Departments of Physics, Biological Sciences and Chemistry, Imperial College London, London SW7 2BW, UK.
Opt Lett. 2004 Mar 15;29(6):584-6. doi: 10.1364/ol.29.000584.
We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells.
我们开发了一种宽场时间分辨成像系统，用于定量成像荧光团的荧光寿命和旋转相关时间。使用偏振分辨成像仪，我们将荧光发射的正交偏振分量同时成像到时间选通增强型电荷耦合器件（ICCD）上。我们通过多孔板中荧光素的旋转相关时间来演示溶剂粘度变化的成像，并将该技术应用于探测活细胞中的微粘度。
