Wang Peng, Mishra Yogeshwar Nath, Bauer Florian J, Gudipati Murthy S, Wang Lihong V
Caltech Optical Imaging Laboratory, Andrew and Peggy Cheng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, Mail Code 138-78, Pasadena, CA, 91125, USA.
Science Division, NASA-Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA, 91109, USA.
Nat Commun. 2025 May 30;16(1):5019. doi: 10.1038/s41467-025-60072-1.
Molecular size plays a crucial role in determining the physical, chemical, and biological properties of substances. However, traditional fluorescence polarization anisotropy methods struggle to capture fast transient processes or provide plane-specific details. To overcome these limitations, we introduce Compressed Ultrafast Planar Polarization Anisotropy Imaging (CUP2AI). This approach combines femtosecond laser-sheet illumination, molecular rotational diffusivity, and compressed sensing to enable real-time, non-invasive, wide-field anisotropy measurements in both liquid and gas phases. CUP2AI captures 2D molecular size mapping in a single acquisition, granting unprecedented insights into dynamic events across various excitation modes (i.e. both one- and two-photon) and environmental conditions. It enables mapping of molecular volume (500 ų-80,000 ų) and hydrodynamic diameter (10 Å-50 Å) based on anisotropy lifetimes. We imaged fluorescein-conjugated dextran in water and polycyclic aromatic hydrocarbons in flames. CUP2AI holds transformative potential for applications ranging from molecular biology and drug design to nanoparticle formation.
分子大小在决定物质的物理、化学和生物学特性方面起着至关重要的作用。然而，传统的荧光偏振各向异性方法难以捕捉快速瞬态过程或提供平面特异性细节。为了克服这些局限性，我们引入了压缩超快平面偏振各向异性成像（CUP2AI）。这种方法结合了飞秒激光片照明、分子旋转扩散率和压缩感知，以实现液相和气相中的实时、非侵入性、宽场各向异性测量。CUP2AI在单次采集时即可捕获二维分子大小图谱，从而对各种激发模式（即单光子和双光子）和环境条件下的动态事件提供前所未有的见解。它能够基于各向异性寿命绘制分子体积（500 ų - 80,000 ų）和流体动力学直径（10 Å - 50 Å）图谱。我们对水中的荧光素偶联葡聚糖和火焰中的多环芳烃进行了成像。CUP2AI在从分子生物学和药物设计到纳米颗粒形成等广泛应用中具有变革性潜力。
