Safavi A, Karimi M A, Hormozi Nezhad M R, Kamali R, Saghir N
Department of Chemistry, Faculty of Sciences, Shiraz University, Shiraz 71454, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2004 Mar;60(4):765-9. doi: 10.1016/S1386-1425(03)00288-9.
A simple, rapid, sensitive and accurate indirect spectrophotometric method for the microdetermination of isoniazid (INH) in pure form and pharmaceutical formulations is developed. The procedure is based on the reaction of copper(II) with isoniazid in the presence of neocuproine (NC). In the presence of neocuproine, copper(II) is reduced easily by isoniazid to a Cu(I)-neocuproine complex, which shows an absorption maximum at 454 nm. By measuring the absorbance of the complex at this wavelength, isoniazid can be determined in the range 0.3-3.5 microgml-1. This method was applied to the determination of isoniazid in pharmaceutical formulation and enabled the determination of the isoniazid in microgram quantities (0.3-3.5 microgml-1). The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official method and demonstrated good accuracy and precision.
建立了一种简单、快速、灵敏且准确的间接分光光度法，用于微量测定纯品异烟肼（INH）及药物制剂中的异烟肼。该方法基于在新亚铜试剂（NC）存在下，铜（II）与异烟肼的反应。在新亚铜试剂存在下，铜（II）很容易被异烟肼还原为Cu（I）-新亚铜试剂络合物，该络合物在454nm处有最大吸收。通过测量该络合物在此波长下的吸光度，可在0.3 - 3.5μg/ml范围内测定异烟肼。该方法应用于药物制剂中异烟肼的测定，能够测定微克量（0.3 - 3.5μg/ml）的异烟肼。药物制剂含量测定的结果与官方方法所得结果相比良好，证明了该方法具有良好的准确性和精密度。
