通过与铜（II）和曙红形成三元配合物分光光度法测定药物制剂中的马来酸氯苯那敏。

Spectrophotometric determination of carbinoxamine maleate in pharmaceutical formulations by ternary complex formation with Cu(II) and eosin.

作者信息

Ramadan A A, Mandil H

机构信息

Department of Chemistry, Faculty of Science, Aleppo University, Syria.

出版信息

Anal Biochem. 2006 Jun 1;353(1):133-7. doi: 10.1016/j.ab.2006.02.020. Epub 2006 Mar 9.

DOI:10.1016/j.ab.2006.02.020

PMID:16574052

Abstract

A simple and sensitive spectrophotometric method was developed for the determination of carbinoxamine maleate in pharmaceutical formulations. The method is based on the formation of a ternary complex, extractable with chloroform, between copper(II), eosin, and carbinoxamine maleate. The absorption spectra of the ternary complexes shows, under optimum conditions, a maxima at 538 nm, with apparent molar absorptive 6.1690 x 10(4) mol(-1) cm(-1), Sandell's sensitives 6.75 x 10(-3) microg cm(-2), and linearity in the concentration range 0.75-10.0 microg ml(-1). The method can be achieved with high accuracy (recovery values, 100 +/- 2%) and precision (with standard deviation 0.029-0.155 and relative standard deviation 3.87-1.55%). The method was again successfully applied, with high accuracy and good precision, for the determination of carbinoxamine maleate in various pharmaceutical formulations (syrup, drops, and tablets).

摘要

建立了一种简单灵敏的分光光度法用于测定药物制剂中的马来酸氯苯那敏。该方法基于铜（II）、曙红与马来酸氯苯那敏形成可被氯仿萃取的三元配合物。在最佳条件下，三元配合物的吸收光谱在538nm处有最大吸收峰，表观摩尔吸光系数为6.1690×10⁴mol⁻¹cm⁻¹，桑德尔灵敏度为6.75×10⁻³μg cm⁻²，浓度范围在0.75 - 10.0μg ml⁻¹内呈线性关系。该方法具有高准确度（回收率为100±2%）和精密度（标准偏差为0.029 - 0.155，相对标准偏差为3.87 - 1.55%）。该方法再次成功地以高准确度和良好精密度用于测定各种药物制剂（糖浆剂、滴剂和片剂）中的马来酸氯苯那敏。