开放阅读框文库计划：基因组学与组学之间的桥梁

ORFeome projects: gateway between genomics and omics.

作者信息

Rual Jean-François, Hill David E, Vidal Marc

机构信息

Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.

出版信息

Curr Opin Chem Biol. 2004 Feb;8(1):20-5. doi: 10.1016/j.cbpa.2003.12.002.

DOI:10.1016/j.cbpa.2003.12.002

PMID:15036152

Abstract

The availability of entire genome sequences is expected to revolutionize the way in which biology and medicine are conducted for years to come. However, achieving this promise still requires significant effort in the areas of gene annotation, cloning and expression of thousands of known and heretofore unknown protein-encoding genes. Traditional technologies of manipulating genes are too cumbersome and inefficient when one is dealing with more than a few genes at a time. Entire libraries composed of all protein-encoding open reading frames (ORFs) cloned in highly flexible vectors will be needed to take full advantage of the information found in any genome sequence. The creation of such ORFeome resources using novel technologies for cloning and expressing entire proteomes constitutes an effective gateway from whole genome sequencing efforts to downstream 'omics' applications.

摘要

预计在未来数年，全基因组序列的可得性将彻底改变生物学和医学的研究方式。然而，要实现这一前景，在基因注释、数千个已知及此前未知的蛋白质编码基因的克隆与表达等领域仍需付出巨大努力。当一次性处理多个基因时，传统的基因操作技术过于繁琐且效率低下。为了充分利用任何基因组序列中发现的信息，将需要由克隆于高度灵活载体中的所有蛋白质编码开放阅读框（ORF）组成的完整文库。利用克隆和表达整个蛋白质组的新技术来创建此类ORFeome资源，构成了从全基因组测序工作通向下游“组学”应用的有效途径。