Alquicer Glenda, Silva-Gómez Adriana B, Peralta Fernando, Flores Gonzalo
Laboratorio de Neuropsiquiatría, Instituto de Fisiología, Universidad Autónoma de Puebla, 14 Sur 6301, CP 72570 Puebla, Mexico.
Int J Dev Neurosci. 2004 Apr;22(2):103-11. doi: 10.1016/j.ijdevneu.2003.12.003.
The neonatal ventral Hippocampus (nVH) lesion in rats has been used as a model to test the hypothesis that early neurodevelopmental abnormalities lead to behavioral changes putatively linked to schizophrenia. The schizophrenic patients tend to social isolation. In addition, considerable evidence from behavioral and neurochemistry studies strongly implicate the dopamine (DA) system and the medial part of the prefrontal cortex (mPFC) in the pathophysiology of the social isolation syndrome. In order to assess effects of the postweaning social isolation (pwSI) on the DA system of the nVH lesions, we investigated the DA content and its metabolite, DOPAC in different limbic subregions in rats postpubertally at postnatal day (P) 78 following nVH lesions at P7 with and without pwSI for 8 weeks. The DA and DOPAC were measured by HPLC with electrochemical detection. The nVH lesion induces increase in the DA content in the hippocampus with no effect in the mPFC, nucleus accumbens and caudate-putamen, while the pwSI induces major increase in the DA content in limbic subregions such as the mPFC, nucleus accumbens and hipocampus with opposite effect in the caudate-putamen. These results suggest that while pwSI has an effect in the postpubertal content of DA in both sham and nVH lesions in rats, the nVH-lesioned rats appear to be affected to a greater extent than the sham animals underscoring the influence of pwSI differences in the development of behaviors in the nVH-lesioned animals.
大鼠新生期腹侧海马（nVH）损伤已被用作一种模型，以检验早期神经发育异常会导致与精神分裂症可能相关的行为变化这一假说。精神分裂症患者往往会出现社交孤立。此外，行为学和神经化学研究的大量证据有力地表明，多巴胺（DA）系统和前额叶皮质内侧部分（mPFC）与社交孤立综合征的病理生理学有关。为了评估断奶后社交孤立（pwSI）对nVH损伤大鼠DA系统的影响，我们研究了在出生后第7天（P7）进行nVH损伤且分别有或无8周pwSI的大鼠在出生后第78天（P78）青春期后不同边缘亚区的DA含量及其代谢产物3,4-二羟基苯乙酸（DOPAC）。通过高效液相色谱电化学检测法测定DA和DOPAC。nVH损伤导致海马中DA含量增加，而对mPFC、伏隔核和尾状核-壳核无影响，而pwSI导致边缘亚区如mPFC、伏隔核和海马中DA含量大幅增加，对尾状核-壳核则产生相反影响。这些结果表明，虽然pwSI对大鼠假手术组和nVH损伤组青春期后DA含量均有影响，但nVH损伤大鼠似乎比假手术动物受到的影响更大，这突出了pwSI差异对nVH损伤动物行为发育的影响。
