Ramezani Mohammad, Hosseinzadeh Hossein, Samizadeh Shokoufeh
Pharmaceutical Research Center, Bu-Ali Research Institute and School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran.
J Ethnopharmacol. 2004 Mar;91(1):167-70. doi: 10.1016/j.jep.2003.12.016.
Activity-guided fractionation of methanol:chloroform (1:1) extract of Zataria multiflora Boiss was performed in order to investigate the active component(s) responsible for antinociceptive activity. Antinociceptive activity was evaluated using hot-plate and writhing tests. Morphine (10 mg/kg, i.p.) and diclofenac (10 mg/kg, i.p.) were used as positive controls. The methanol-chloroform (1:1) extract of the aerial parts of the plants was prepared and partitioned between methanol-water (9:1) and n-hexane. The more active hydroalcoholic fraction was concentrated and further partitioned between methanol-water (3:2) and chloroform. The hydroalcoholic layer showed a dose-dependent and significant activity compared to morphine and diclofenac. Further fractionation of the hydroalcoholic fraction on silica gel gave six fractions. Three fractions at 100 mg/kg showed activity comparable to those of morphine.
为了研究负责抗伤害感受活性的活性成分，对百里香（Zataria multiflora Boiss）的甲醇：氯仿（1:1）提取物进行了活性导向分级分离。使用热板法和扭体试验评估抗伤害感受活性。吗啡（10mg/kg，腹腔注射）和双氯芬酸（10mg/kg，腹腔注射）用作阳性对照。制备植物地上部分的甲醇-氯仿（1:1）提取物，并在甲醇-水（9:1）和正己烷之间进行分配。活性更强的水醇馏分被浓缩，并进一步在甲醇-水（3:2）和氯仿之间进行分配。与吗啡和双氯芬酸相比，水醇层显示出剂量依赖性的显著活性。水醇馏分在硅胶上进一步分级得到六个馏分。三个100mg/kg的馏分显示出与吗啡相当的活性。
