迷迭香和香芹酚标准化提取物对乙酰氨基酚诱导的大鼠肝毒性的预防作用：迷迭香、香芹酚与肝毒性
The Preventive Effects of Standardized Extract of and Carvacrol on Acetaminophen-Induced Hepatotoxicity in Rat: - Carvacrol and Hepatotoxicity.
作者信息
Mohebbati Reza, Paseban Maryam, Beheshti Farimah, Soukhtanloo Mohammad, Shafei Mohammad Naser, Rakhshandeh Hasan, Rad Abolfazl Khajavi
机构信息
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
J Pharmacopuncture. 2018 Dec;21(4):249-257. doi: 10.3831/KPI.2018.21.028. Epub 2018 Dec 31.
OBJECTIVES
The hepatotoxicity induced by Acetaminophen (AAP) mostly mediated by effect on oxidative stress parameters. The (Z.M) is an herbal medicine with well-known antioxidant effect. The aim of this study is investigation of preventive effects of Z.M and Carvacrol (CAR) on AAP-induced hepatotoxicity in rats.
METHODS
Rats were randomly divided into four groups including: 1) Control, 2) Acetaminophen (AAP), 3) and 4) CAR. The saline, Z.M (200 mg/kg) and CAR (20 mg/kg) were administrated orally for 6 days, after that AAP (600 mg/kg) was administrated in the 7 day. Blood sampling was performed on the first and last days. Also, the liver tissue was removed for evaluation of Malondyaldehide (MDA), Thiol content, Superoxide dismutase (SOD) and Catalase (CAT). Total Protein (tPro), Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT) and Alkaline Phosphatase (ALP) in liver tissue were evaluated. The changes (Δ) of enzymes activities were presented.
RESULTS
The Δ GOT, Δ GPT and Δ ALP in CAR group significantly decreased compared to AAP group (P < 0.01 to P < 0.001) and Δ GPT in Z.M group was significantly reduced in comparison with AAP group (P < 0.05). Also, MDA, Thiol, SOD and CAT levels in treated groups were attenuated compared to AAP group (P < 0.05 to P < 0.001).
CONCLUSION
Z.M and CAR have a powerful hepatoprotective effect. CAR is more effective than Z.M. Based on the results. Z.M and CAR could be potent supplementary agents against hepatotoxicity of AAP in patients.
目的
对乙酰氨基酚（AAP）诱导的肝毒性大多由其对氧化应激参数的影响介导。Z.M是一种具有著名抗氧化作用的草药。本研究的目的是调查Z.M和香芹酚（CAR）对AAP诱导的大鼠肝毒性的预防作用。
方法
将大鼠随机分为四组，包括：1）对照组，2）对乙酰氨基酚（AAP）组，3）和4）CAR组。连续6天口服生理盐水、Z.M（200毫克/千克）和CAR（20毫克/千克），之后在第7天给予AAP（600毫克/千克）。在第一天和最后一天进行采血。同时，取出肝脏组织以评估丙二醛（MDA）、巯基含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。评估肝脏组织中的总蛋白（tPro）、谷草转氨酶（GOT）、谷丙转氨酶（GPT）和碱性磷酸酶（ALP）。呈现酶活性的变化（Δ）。
结果
与AAP组相比，CAR组的ΔGOT、ΔGPT和ΔALP显著降低（P<0.01至P<0.001），与AAP组相比，Z.M组的ΔGPT显著降低（P<0.05）。此外，与AAP组相比，治疗组的MDA、巯基、SOD和CAT水平降低（P<0.05至P<0.001）。
结论
Z.M和CAR具有强大的肝脏保护作用。基于结果，CAR比Z.M更有效。Z.M和CAR可能是预防患者AAP肝毒性的有效补充剂。
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