Tanaka Yasukazu, Han Huiwan, Hagishita Tairo, Fukui Fumiko, Liu Guoquan, Ando Susumu
Neuronal Function Research Group, Division of Neuroscience and Brain Function, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan.
Neurosci Lett. 2004 Feb 26;357(1):9-12. doi: 10.1016/j.neulet.2003.11.062.
The effects of alpha-sialylcholesterol (alpha-SC), a synthetic ganglioside analogue, on synaptic neurotransmission were studied using in vivo microdialysis technique. Application of alpha-SC through a microdialysis probe enhanced high potassium-evoked release of acetylcholine and glutamate in the hippocampal CA3 region of Wistar rats. The experiments using synaptosomes and FM1-43, a fluorescent styryl dye used for studies of neurotransmitter release mechanisms, showed that alpha-SC increased depolarization-induced loss of dye but it did not evoke the dye loss at resting condition. These results indicate that alpha-SC promotes a depolarization-induced exocytotic neurotransmitter release in the brain under in vivo conditions. Application of alpha-SC increased the level of glutamate but not that of acetylcholine, suggesting that alpha-SC affects spontaneous glutamate release and/or transport system at the brain region.
使用体内微透析技术研究了合成神经节苷脂类似物α-唾液酸胆固醇（α-SC）对突触神经传递的影响。通过微透析探针施加α-SC可增强Wistar大鼠海马CA3区高钾诱发的乙酰胆碱和谷氨酸释放。使用突触体和FM1-43（一种用于研究神经递质释放机制的荧光苯乙烯基染料）进行的实验表明，α-SC增加了去极化诱导的染料损失，但在静息状态下不会引起染料损失。这些结果表明，在体内条件下，α-SC促进大脑中去极化诱导的胞吐性神经递质释放。施加α-SC可增加谷氨酸水平，但不会增加乙酰胆碱水平，这表明α-SC会影响大脑区域的自发性谷氨酸释放和/或转运系统。
