Morioka Takamitsu, Suzui Masumi, Nabandith Viengvansay, Inamine Morihiko, Aniya Yoko, Nakayama Takashi, Ichiba Toshio, Mori Hideki, Yoshimi Naoki
Tumor Pathology, Faculty of Medicine, University of the Ryukyus, 207 Uehara Nishihara-cho, Okinawa 903-0215, Japan.
Cancer Lett. 2004 Mar 18;205(2):133-41. doi: 10.1016/j.canlet.2003.10.002.
The modifying effect of dietary Peucedanum japonicum (PJ), which is a traditional herb in the Ryukyu Islands and is an anti-oxidant, on azoxymethane (AOM)-induced rat colon carcinogenesis was examined. Male F344 rats were divided into six groups: rats in groups 1-4 were given subcutaneous injection of AOM (20 mg/kg body weight) once a week for 2 weeks. Rats in groups 2, 3 and 4 were fed the diets containing 0.2 and 1% PJ and 0.025% chlorogenic acid, respectively. We observed modification of the preneoplastic lesions of both aberrant crypt foci (ACF) and beta-catenin accumulated crypts (BCAC) in colon carcinogenesis, microscopically and immunohistochemically. The numbers of ACF consisting of more than four aberrant crypts per rat in groups 2 (3.2+/-1.7) and 3 (3.0+/-3.2) were significantly lower than that of group 1 (10.8+/-4.9; P<0.05, respectively). The mean number of BCAC in both groups 2 (0.88+/-0.48/cm2/rat) and 3 (0.81+/-0.34/cm2/rat) was significantly lower than that in group 1 (2.13+/-0.54/cm2/rat; P < 0.0001, respectively). In addition, proliferating cell nuclear antigen labeling indices in group 2 (10.98+/-2.03) and group 3 (9.85+/-2.62) were significantly lower than that in group 1 (14.87+/-3.93; P < 0.001 and P < 0.0001, respectively). These findings indicate that PJ inhibits both ACF formation and accumulation of beta-catenin, and that PJ also reduces the cell proliferation activity, suggesting that PJ may have chemopreventive potential for colon carcinogenesis.
研究了作为琉球群岛传统草药且具有抗氧化作用的饮食中峨参（PJ）对氧化偶氮甲烷（AOM）诱导的大鼠结肠癌发生的修饰作用。雄性F344大鼠分为六组：第1 - 4组大鼠每周皮下注射一次AOM（20 mg/kg体重），共2周。第2、3和4组大鼠分别喂食含0.2%和1% PJ以及0.025%绿原酸的饮食。我们通过显微镜和免疫组织化学观察了结肠癌发生过程中异常隐窝灶（ACF）和β-连环蛋白积聚隐窝（BCAC）这两种癌前病变的修饰情况。第2组（3.2±1.7）和第3组（3.0±3.2）中每只大鼠由四个以上异常隐窝组成的ACF数量显著低于第1组（10.8±4.9；P分别<0.05）。第2组（0.88±0.48/cm²/大鼠）和第3组（0.81±0.34/cm²/大鼠）中BCAC的平均数量均显著低于第1组（2.13±0.54/cm²/大鼠；P分别<0.0001）。此外，第2组（10.98±2.03）和第3组（9.85±2.62）的增殖细胞核抗原标记指数显著低于第1组（14.87±3.93；P分别<0.001和P<0.0001）。这些发现表明PJ抑制ACF形成和β-连环蛋白积聚，并且PJ还降低细胞增殖活性，提示PJ可能对结肠癌发生具有化学预防潜力。
