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水飞蓟宾对人结直肠癌细胞的诱导凋亡和抑制侵袭作用及其机制研究

Aerial Parts of Peucedanum chenur Have Anti-Cancer Properties through the Induction of Apoptosis and Inhibition of Invasion in Human Colorectal Cancer Cells.

机构信息

Research Center for Molecular Medicine and Genetics, Hamadan University of Medical Sciences, Hamadan, Iran.

Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

出版信息

Iran Biomed J. 2020 Sep;24(5):314-23. doi: 10.29252/ibj.24.5.309. Epub 2020 Mar 4.

DOI:10.29252/ibj.24.5.309
PMID:32429645
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7392135/
Abstract

BACKGROUND

The Peucedanum species have many pharmacological effects due to the presence of coumarins, flavonoids, phenolic compounds, and essential fatty acids in these species. In this study, for the first time, the anticancer activity of Peucedanum chenur methanolic extract via the induction of apoptosis and inhibition of invasion in HCT-116 human colon cancer cells was investigated.

METHODS

P. chenur methanolic extract effect on HCT-116 cells viability and antioxidant activity were evaluated using MTT assay, 1,1-Diphenyl-2-picrylhydrazyl, and iron chelating tests, respectively. Changes in mRNA expression level in a panel of relevant genes were assessed by the quantitative real-time PCR. Also, apoptosis was assessed by cell cycle analysis and Annexin V/PI (propidium iodide) method, and the effect on cell migration was tested using scratch test.

RESULTS

P. chenur methanolic extract increased significantly the expression of BAX while decreased the expression of BCL-2, AKT1, FAK, RhoA, and matrix metalloproteinase (MMP) genes compared to the control group. BAX/BCL-2 ratio and apoptosis elevated, whereas cell migration reduced significantly. Besides, our extract showed an appropriate antioxidant activity.

CONCLUSION

P. chenur may be introduced as a new chemopreventive agent in medicine due to its notable power in terms of induction of apoptosis and inhibition of invasion.

摘要

背景

由于泽芹属植物中存在香豆素、类黄酮、酚类化合物和必需脂肪酸，因此具有许多药理作用。在这项研究中，首次研究了泽芹甲醇提取物通过诱导细胞凋亡和抑制 HCT-116 人结肠癌细胞侵袭来抑制癌症的活性。

方法

使用 MTT 测定法、1,1-二苯基-2-苦基肼（1,1-Diphenyl-2-picrylhydrazyl）和铁螯合试验分别评估了 P. chenur 甲醇提取物对 HCT-116 细胞活力和抗氧化活性的影响。通过定量实时 PCR 评估相关基因表达谱中基因表达水平的变化。通过细胞周期分析和 Annexin V/PI（碘化丙啶）法评估细胞凋亡，通过划痕试验测试细胞迁移的影响。

结果

与对照组相比，P. chenur 甲醇提取物显著增加了 BAX 的表达，同时降低了 BCL-2、AKT1、FAK、RhoA 和基质金属蛋白酶（MMP）基因的表达。BAX/BCL-2 比值和凋亡显著增加，而细胞迁移明显减少。此外，我们的提取物表现出适当的抗氧化活性。

结论

由于泽芹提取物在诱导细胞凋亡和抑制侵袭方面具有显著的作用，因此可以作为一种新的化学预防药物引入医学领域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/ccb56d563e4b/ibj-24-314-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/2faf982777ab/ibj-24-314-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/4c19697d983e/ibj-24-314-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/ebc11138206c/ibj-24-314-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/bcebcf391585/ibj-24-314-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/ccb56d563e4b/ibj-24-314-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/2faf982777ab/ibj-24-314-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/4c19697d983e/ibj-24-314-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/ebc11138206c/ibj-24-314-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/bcebcf391585/ibj-24-314-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18aa/7392135/ccb56d563e4b/ibj-24-314-g005.jpg

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