Pathogen-derived resistance in potato to Potato virus Y-aspects of stability and biosafety under field conditions.

Plants of three different potato cultivars/lines were transformed via Agrobacterium tumefaciens with a truncated NIb gene of a necrotic strain of Potato virus Y (PVY(N)) which had been C-terminally fused to enhanced blue-fluorescing protein. Resistance of the resulting transgenic clones was evaluated under glass house conditions using an NTN-strain of PVY. Four clones with the highest levels of resistance were chosen for further experiments. Their type of resistance was either recovery or extreme resistance. These clones and their resistance types were also characterised at the molecular level. Mechanisms other than post-transcriptional gene silencing seemed to be involved in the resistance which was not dependent on sequence homology between transgene and challenging virus. Stability of resistance was tested under field conditions. The plants usually became infected with PVY. Tubers of the clone with extreme resistance did not recover from infection whereas those from clones with the recovery type did. No influence of transgenic potatoes was apparent on aphid population numbers in test plots. Recombination events could not be detected at the RNA level between transgene and challenging virus.