Smit Martha S
Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa.
Trends Biotechnol. 2004 Mar;22(3):123-9. doi: 10.1016/j.tibtech.2004.01.012.
Epoxide hydrolases are useful catalysts for the hydrolytic kinetic resolution of epoxides, which are sought after intermediates for the synthesis of enantiopure fine chemicals. The epoxide hydrolases from Aspergillus niger and from the basidiomycetous yeasts Rhodotorula glutinis and Rhodosporidium toruloides have demonstrated potential as versatile, user friendly biocatalysts for organic synthesis. A recombinant A. niger epoxide hydrolase, produced by an overproducing A. niger strain, is already commercially available and recombinant yeast epoxide hydrolases expressed in Escherichia coli have shown excellent results. Within the vast body of activity information on the one hand and gene sequence information on the other hand, the epoxide hydrolases from the Rhodotorula spp. and A. niger stand out because we have sequence information as well as activity information for both the wild-type and recombinant forms of these enzymes.
环氧化物水解酶是用于环氧化物水解动力学拆分的有用催化剂，环氧化物是合成对映体纯精细化学品所需的中间体。黑曲霉以及担子菌酵母粘红酵母和球形红冬孢酵母中的环氧化物水解酶已显示出作为用于有机合成的多功能、用户友好型生物催化剂的潜力。由高产黑曲霉菌株产生的重组黑曲霉环氧化物水解酶已在市场上销售，并且在大肠杆菌中表达的重组酵母环氧化物水解酶已显示出优异的结果。在一方面大量的活性信息和另一方面基因序列信息中，红酵母属和黑曲霉的环氧化物水解酶脱颖而出，因为我们拥有这些酶的野生型和重组形式的序列信息以及活性信息。
